Metabolic extract ofFusarium oxysporuminduces histopathologic alterations and apoptosis in the skin of Wistar rats
| Autor: | Rosalva Thereza Meurer, Terezinha Inez Estivalet Svidzinski, Aline Vansan Marangon, Tânia Pereira Salci, Marilda da Cruz Fernandes, Luzmarina Hernandes |
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| Rok vydání: | 2009 |
| Předmět: |
Male
medicine.medical_specialty Pathology Injections Intradermal H&E stain Apoptosis Inflammation Dermatology Collagen Type I chemistry.chemical_compound Immune system Fusarium Dermis In Situ Nick-End Labeling Animals Humans Medicine Mast Cells Rats Wistar Sirius Red TUNEL assay business.industry Mycotoxins Rats Disease Models Animal Collagen Type III medicine.anatomical_structure Mycoses chemistry Histopathology medicine.symptom business |
| Zdroj: | International Journal of Dermatology. 48:697-703 |
| ISSN: | 1365-4632 0011-9059 |
| Popis: | Background Most patients with Fusarium infection present with affection of the tegumentary system, with the presence of necrotic and/or inflammatory lesions associated with pain. Aim To evaluate the effect of the intradermal application of a metabolic extract of Fusarium oxysporum in the skin of Wistar rats. Methods The extract was obtained from fungal cultivation in Czapek-Dox medium. It was sterilized by filtration through a Millipore membrane, and injected (0.5 mg/mL) intradermally into the skin of rats, which were killed 3, 6, 12, and 24 h after inoculation. Skin specimens were placed in paraffin and stained using hematoxylin and eosin, and toluidine blue, for the evaluation of the inflammatory response, and Sirius red for the quantification of collagen. Terminal deoxynucleotidyl transferase-mediated UTP nick end labelling (TUNEL) was used for the identification of apoptosis. Tissue reactions were graded and compared over time and compartment. Results The inflammatory reaction reached a peak at 12 h for both the dermis and subcutaneous region, being graded as moderate and moderate to severe, respectively. There was an influx of granulocytes, lymphocytes, and macrophages. A significant increase in the number of mast cells, as well as the presence of hyperemic vessels and apoptotic bodies, was observed. There was TUNEL staining in keratinocytes, fibroblasts, endothelial cells, muscles, and in the cells of the inflammatory infiltrate. There was a significant decrease in the area occupied by collagen after 12 h. Conclusions The extract induced histopathologic alterations in the skin, probably as a result of the presence of active toxic metabolites. |
| Databáze: | OpenAIRE |
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