Classifications of atherosclerotic plaque components with T1 and T2* mapping in 11.7 T MRI
| Autor: | Isabel Gonçalves, Lena Sundius, Adnan Bibic, My Truong, Finn Lennartsson, Roger Siemund, Ana Persson, Johan Wassélius, René in ‘t Zandt |
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| Rok vydání: | 2020 |
| Předmět: |
Pathology
ROI region of interest R895-920 Plaque components FOV field of view 030218 nuclear medicine & medical imaging Medical physics. Medical radiology. Nuclear medicine 0302 clinical medicine TBS tris-buffered saline HRP horse radish peroxidase OCT optimal cutting temperature 11.7T 11.7 Tesla RF radio frequency LRNC lipid rich necrotic core TE echo time medicine.diagnostic_test GE3D gradient echo three dimensional Classification Carotid plaque TR repetition time 030220 oncology & carcinogenesis CTA computed tomography angiography Internal carotid artery ms millisecond medicine.medical_specialty TIA transient ischemic attack 3T 3 Tesla IPH intra-plaque hemorrhage T2*maps ICA internal carotid artery Article 11.7 T MRI 03 medical and health sciences Region of interest medicine.artery medicine Radiology Nuclear Medicine and imaging ComputingMethodologies_COMPUTERGRAPHICS T1 maps T1w T1 weighted business.industry Magnetic resonance imaging Histology Gold standard (test) Atherosclerosis T2*w T2 star weighted Staining FA flip angle CI confidence interval Histopathology BSA bovine serum albumin business SD standard deviation MRI magnetic resonance imaging Ex vivo |
| Zdroj: | European Journal of Radiology Open European Journal of Radiology Open, Vol 8, Iss, Pp 100323-(2021) |
| ISSN: | 2352-0477 |
| Popis: | Graphical abstract Highlights • Ex vivo MRI in 11.7 T with T1/T2* maps, is a non-destructive method to study carotid plaque content with good visual agreement with histology. • Quadratic discriminant analysis on ROI data from T1 and T2* maps, is a promising method to classify plaque content. • Classification is more challenging in plaques with hemorrhage or inflammation. Background and aims Histopathology is the gold standard for analysis of atherosclerotic plaques but has drawbacks due to the destructive nature of the method. Ex vivo MRI is a non-destructive method to image whole plaques. Our aim was to use quantitative high field ex vivo MRI to classify plaque components, with histology as gold standard. Methods Surgically resected carotid plaques from 12 patients with recent TIA or stroke were imaged at 11.7 T MRI. Quantitative T1/T2* mapping sequences and qualitative T1/T2* gradient echo sequences with voxel size of 30 × 30 × 60 μm3 were obtained prior to histological preparation, sectioning and staining for lipids, inflammation, hemorrhage, and fibrous tissue. Regions of interest (ROI) were selected based on the histological staining at multiple levels matched between histology and MRI. The MRI parameters of each ROI were then analyzed with quadratic discriminant analysis (QDA) for classification. Results A total of 965 ROIs, at 70 levels matched between histology and MRI, were registered based on histological staining. In the nine plaques where three or more plaque components were possible to co-localize with MRI, the mean degree of misclassification by QDA was 16.5 %. One of the plaques contained mostly fibrous tissue and lipids and had no misclassifications, and two plaques mostly contained fibrous tissue. QDA generally showed good classification for fibrous tissue and lipids, whereas plaques with hemorrhage and inflammation had more misclassifications. Conclusion 11.7 T ex vivo high field MRI shows good visual agreement with histology in carotid plaques. T1/T2* maps analyzed with QDA is a promising non-destructive method to classify plaque components, but with a higher degree of misclassifications in plaques with hemorrhage or inflammation. |
| Databáze: | OpenAIRE |
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