Effect of Transcatheter Arterial Embolization on Levels of Hypoxia-inducible Factor-1α in Rabbit VX2 Liver Tumors
| Autor: | Mary F. Mulcahy, G. Kenneth Haines, Thomas K. Rhee, Andrew C. Larson, Tatjana Paunesku, Reed A. Omary, Riad Salem, Gayle E. Woloschak, Joseph Y. Young, Laura Kulik, Kent T. Sato |
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| Rok vydání: | 2007 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.drug_class medicine.medical_treatment Monoclonal antibody Liver Neoplasms Experimental Cell Line Tumor medicine Animals Radiology Nuclear Medicine and imaging Embolization Chemoembolization Therapeutic medicine.diagnostic_test business.industry Arterial Embolization Magnetic resonance imaging Blood flow Hypoxia-Inducible Factor 1 alpha Subunit medicine.disease Staining Disease Models Animal Hypoxia-inducible factors Hepatocellular carcinoma Feasibility Studies Rabbits Cardiology and Cardiovascular Medicine business |
| Zdroj: | Journal of Vascular and Interventional Radiology. 18:639-645 |
| ISSN: | 1051-0443 |
| DOI: | 10.1016/j.jvir.2007.02.031 |
| Popis: | Purpose To test the hypothesis that transcatheter arterial embolization (TAE) of VX2 rabbit liver tumors increases the expression of hypoxia-inducible factor-1α (HIF-1α), a transcription factor that regulates the expression of pro-angiogenic genes. Materials and Methods VX2 tumors were implanted in the livers of eight New Zealand white rabbits. Once tumor growth was seen at T2-weighted turbo spin-echo magnetic resonance (MR) imaging, four of the eight rabbits underwent TAE with 45–150-μm polyvinyl alcohol particles. The remaining four rabbits served as non-TAE controls. The TAE end point was stasis of antegrade blood flow. All rabbits were sacrificed for tumor harvest 2 hours after TAE. Tumor tissue and corresponding normal liver tissue in each rabbit liver were stained with anti-human HIF-1α monoclonal antibody and reviewed with light microscopy. Percentages of stained viable tumor and normal liver cells were compared by using the Mann-Whitney U test (α = 0.05). Results In eight rabbits with 24 discrete liver tumors, the mean percentage (±standard deviation) of positive HIF-1α–stained cells in the TAE group was greater than that in the control group (19% ± 7.0 vs 12% ± 8.0, respectively) ( P = .05). Normal liver tissue in both the TAE and control groups showed no HIF-1α staining. Conclusion Although HIF-1α is not expressed in normal rabbit liver parenchyma—even after TAE—HIF-1α expression is present in implanted VX2 rabbit liver tumors and significantly increased in lesions that have undergone embolization. |
| Databáze: | OpenAIRE |
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