The effect of surface energy, adsorbed RGD peptides and fibronectin on the attachment and spreading of cells on multiwalled carbon nanotube papers
| Autor: | Brigitte Delord, Elisabeth Sellier, Christine Labrugère, Jean-Paul Salvetat, Sébastien Gounel, Philippe Poulin, Guillaume Vidal, Wilfrid Neri, Christèle Bartholome, Marie-Christine Durrieu, Olivier Roubeau, Isabelle Ly, Joëlle Amédée |
|---|---|
| Přispěvatelé: | Centre de recherches Paul Pascal (CRPP), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie de la Matière Condensée de Bordeaux (ICMCB), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Université de Bordeaux (UB), Centre de Ressources en Microscopie Electronique et Microanalyse, Université de Bordeaux (UB), Biomateriaux et Reparation Tissulaire, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
chemistry.chemical_classification
0303 health sciences Nanotube Materials science biology Substrate (chemistry) Peptide [CHIM.MATE]Chemical Sciences/Material chemistry 02 engineering and technology General Chemistry Adhesion 021001 nanoscience & nanotechnology 11058 (ICMCB) Fibronectin 03 medical and health sciences Membrane chemistry Biochemistry Biophysics biology.protein General Materials Science Viability assay 0210 nano-technology Cell adhesion 030304 developmental biology |
| Zdroj: | Carbon Carbon, Elsevier, 2011, 49 (7), pp.2318-2333. ⟨10.1016/j.carbon.2011.01.064⟩ Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0008-6223 |
| DOI: | 10.1016/j.carbon.2011.01.064⟩ |
| Popis: | 16 páginas, 15 figuras, 2 tablas.-- et al. We report that hydrophilic oxidized multiwalled carbon nanotube papers (CNPs) were efficient substrates for attachment, spreading, and proliferation of MG-63 cells. Complete spreading occurred in the first 4 h of culture even without complete serum or pre-adsorbed adhesion proteins such as collagen or fibronectin. By contrast, the density of adherent cell on hydrophobic CNPs was low. Spreading did not happen after 24 h of culture in absence of serum proteins and growth factors on such papers. Cell viability concomitantly decreased. We also observed that a short RGD peptide, designed to adsorb on both hydrophobic and hydrophilic CNPs, enhanced adhesion, spreading, and proliferation only on hydrophilic CNPs. By contrast, adsorbed fibronectin triggers cell adhesion and spreading on both types of CNPs. Our results show that the imitation of tissue basal membranes with the nanofibrous structure of CNPs is insufficient to trigger the proliferation and spread of cells. Chemical cues are also needed for cells to spread. CNPs, however, offer a better substrate for adhesion protein absorption than do non-porous polymers. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect