Genome-wide analysis of proline-rich extension-like receptor protein kinase (PERK) in Brassica rapa and its association with the pollen development
| Autor: | Wang Chenggang, Jianghua Song, Jie Li, Jian Wang, Tang Xiaoyan, Wang Hao, Guohu Chen, Lei Zhang, Hou Jinfeng, Yuan Lingyun |
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| Rok vydání: | 2020 |
| Předmět: |
lcsh:QH426-470
Proline lcsh:Biotechnology Male sterile Comparative genome analysis Paralogous Gene Biology Genome Transcriptomic analysis Transcriptome Evolution Molecular lcsh:TP248.13-248.65 Gene duplication Brassica rapa Genetics Gene Phylogeny Synteny Plant Proteins Meiosis II Gene Expression Profiling Proline-rich receptor-like protein kinase lcsh:Genetics Pollen Protein Kinases Genome Plant Biotechnology Genome-Wide Association Study Research Article |
| Zdroj: | BMC Genomics BMC Genomics, Vol 21, Iss 1, Pp 1-13 (2020) |
| ISSN: | 1471-2164 |
| Popis: | Background Proline-rich extension-like receptor protein kinases (PERKs) are an important class of receptor kinases located in the plasma membrane, most of which play a vital role in pollen development. Results Our study identified 25 putative PERK genes from the whole Brassica rapa genome (AA). Phylogenetic analysis of PERK protein sequences from 16 Brassicaceae species divided them into four subfamilies. The biophysical properties of the BrPERKs were investigated. Gene duplication and synteny analyses and the calculation of Ka/Ks values suggested that all 80 orthologous/paralogous gene pairs between B. rapa and A. thaliana, B. nigra and B. oleracea have experienced strong purifying selection. RNA-Seq data and qRT-PCR analyses showed that several BrPERK genes were expressed in different tissues, while some BrPERKs exhibited high expression levels only in buds. Furthermore, comparative transcriptome analyses from six male-sterile lines of B. rapa indicated that 7 BrPERK genes were downregulated in all six male-sterile lines. Meanwhile, the interaction networks of the BrPERK genes were constructed and 13 PERK coexpressed genes were identified, most of which were downregulated in the male sterile buds. Conclusion Combined with interaction networks, coexpression and qRT-PCR analyses, these results demonstrated that two BrPERK genes, Bra001723.1 and Bra037558.1 (the orthologs of AtPERK6 (AT3G18810)), were downregulated beginning in the meiosis II period of male sterile lines and involved in anther development. Overall, this comprehensive analysis of some BrPERK genes elucidated their roles in male sterility. |
| Databáze: | OpenAIRE |
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