Identification of infiltrating immune cell subsets and heterogeneous macrophages in the lesion microenvironment of hepatic cystic echinococcosis patients with different cyst viability
Autor: | Bo Ran, Wending Li, Hao Wen, Yingmei Shao, Aimaiti Yasen, Zongding Wang, Tiemin Jiang, Abudusalamu Aini, Tuerganaili Aji |
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Rok vydání: | 2021 |
Předmět: |
CD4-Positive T-Lymphocytes
Liver Cirrhosis Echinococcosis Hepatic Pathology medicine.medical_specialty Veterinary (miscellaneous) CD19 Lesion Immune system medicine Humans Macrophage Cyst B-Lymphocytes Extracellular Matrix Proteins biology Cysts CD68 business.industry Macrophages medicine.disease M2 Macrophage Phenotype Infectious Diseases Insect Science biology.protein Parasitology medicine.symptom business |
Zdroj: | Acta Tropica. 221:106029 |
ISSN: | 0001-706X |
Popis: | Human cystic echinococcosis (CE) is characterized by lesion microenvironment formation through gathering various immune cells, including macrophages. However, immune cell subsets and heterogeneous macrophages in CE lesion microenvironment are poorly defined. Massive infiltrating immune cells formed lesion microenvironment, among which CD4+T cells and CD19+B cells were predominant and CD68+ macrophages were more evident in patients with active cysts. Different degrees of liver fibrosis was observed in Peri-Lesion (PL) liver samples, which was more evident in patients with active cysts. Expression of both M1 and M2 macrophage markers was significantly increased in PL liver samples. Importantly, elevation of M1 macrophage markers was more obvious in patients with inactive cysts, whereas M2 macrophage markers represented dominant macrophage phenotype in patients with active cysts. Additionally, macrophage-derived MIF, TGF-β1 and ECM1 were also expressed at higher level in CE lesion microenvironment of patients with active cysts. Moreover, MIF was evidently enhanced in the serum of hepatic CE patients, which was also predominant in patients with active cysts. Correlation analysis demonstrated positive correlation between expression of macrophage-derived cytokines and liver fibrosis degree. Heterogeneous macrophages may play significant roles in liver fibrosis of CE lesion microenvironment through producing pro-fibrogenic cytokines. |
Databáze: | OpenAIRE |
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