Fibrinogen alpha genes: conservation of bipartite transcripts and carboxy-terminal-extended alpha subunits in vertebrates
| Autor: | Yan Cao, Kathe M. Hertzberg, Gerd Grieninger, Yiping Fu |
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| Rok vydání: | 1995 |
| Předmět: |
Transcription
Genetic Stereochemistry Protein subunit Molecular Sequence Data Biology Homology (biology) Conserved sequence Exon Sequence Homology Nucleic Acid Genetics Animals Humans Amino Acid Sequence Conserved Sequence chemistry.chemical_classification Base Sequence Nucleic acid sequence Intron Fibrinogen Molecular biology Amino acid Rats chemistry Vertebrates Bipartite graph Rabbits Chickens Papio |
| Zdroj: | Genomics. 30(1) |
| ISSN: | 0888-7543 |
| Popis: | All three well-studied subunits of the clotting protein fibrinogen ({alpha}, {beta}, {gamma}) share N-terminal structural homologies, but until recently only the {beta} and {gamma} chains were recognized as having similar globular C-termini. With the discovery of an extra exon in the human fibrinogen {alpha} gene (exon VI), a minor form of the {alpha} subunit ({alpha}{sub E}) with an extended {beta}- and {gamma}-like C-terminus has been identified. In the present study, the polymerase chain reaction has been used to identify sequences that encode counterparts to {alpha}{sub E} in chicken, rabbit, rat, and baboon. The basic six-exon structure of the fibrinogen {alpha} genes is shown to be conserved among mammals and birds, as are the intron positions. Bipartite transcripts - still bearing an intron prior to the last exon - are found among the products of the various vertebrate fibrinogen {alpha} genes. The last exon represents the largest conserved segment of the gene and, in each species examined, encodes exactly 236 amino acids. The C-termini of these {alpha}{sub E} chains align without a single gap and are between 76 and 99% identical. Since the exon VI-encoded domain of {alpha}{sub E} is as well conserved as the corresponding regions of the {beta} andmore » {gamma} chains, it follows that it is equally important and that {alpha}{sub E}-fibrinogen plays a vital, if as-yet unrecognized physiological role. 21 refs., 7 figs., 1 tab.« less |
| Databáze: | OpenAIRE |
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