TissueCypher™: A systems biology approach to anatomic pathology
| Autor: | Lansing D. Taylor, Xuan M Nguyen, Jeffrey W. Prichard, Kathleen A Repa, Jon M. Davison, Rebecca J. Critchley-Thorne, Jinhong Li, Lia M Reese, Tyler Foxwell, Bruce B. Campbell |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Pathology Stromal cell quantitative image analysis reactive atypia Health Informatics lcsh:Computer applications to medicine. Medical informatics computer vision Pathology and Forensic Medicine Barrett's esophagus Technical Note medicine Atypia lcsh:Pathology Barrett's esophagus biomarkers computer vision digital pathology high-grade dysplasia multiplexed immunofluorescence quantitative image analysis reactive atypia stromal cells TissueCypher stromal cells business.industry biomarkers Digital pathology Anatomical pathology medicine.disease Computer Science Applications high-grade dysplasia TissueCypher™ Dysplasia Immunohistochemistry Biomarker (medicine) lcsh:R858-859.7 multiplexed immunofluorescence digital pathology business lcsh:RB1-214 |
| Zdroj: | Journal of Pathology Informatics, Vol 6, Iss 1, Pp 48-48 (2015) Journal of Pathology Informatics |
| ISSN: | 2153-3539 |
| Popis: | Background: Current histologic methods for diagnosis are limited by intra- and inter-observer variability. Immunohistochemistry (IHC) methods are frequently used to assess biomarkers to aid diagnoses, however, IHC staining is variable and nonlinear and the manual interpretation is subjective. Furthermore, the biomarkers assessed clinically are typically biomarkers of epithelial cell processes. Tumors and premalignant tissues are not composed only of epithelial cells but are interacting systems of multiple cell types, including various stromal cell types that are involved in cancer development. The complex network of the tissue system highlights the need for a systems biology approach to anatomic pathology, in which quantification of system processes is combined with informatics tools to produce actionable scores to aid clinical decision-making. Aims: Here, we describe a quantitative, multiplexed biomarker imaging approach termed TissueCypher™ that applies systems biology to anatomic pathology. Applications of TissueCypher™ in understanding the tissue system of Barrett's esophagus (BE) and the potential use as an adjunctive tool in the diagnosis of BE are described. Patients and Methods: The TissueCypher™ Image Analysis Platform was used to assess 14 epithelial and stromal biomarkers with known diagnostic significance in BE in a set of BE biopsies with nondysplastic BE with reactive atypia (RA, n = 22) and Barrett's with high-grade dysplasia (HGD, n = 17). Biomarker and morphology features were extracted and evaluated in the confirmed BE HGD cases versus the nondysplastic BE cases with RA. Results: Multiple image analysis features derived from epithelial and stromal biomarkers, including immune biomarkers and morphology, showed significant differences between HGD and RA. Conclusions: The assessment of epithelial cell abnormalities combined with an assessment of cellular changes in the lamina propria may serve as an adjunct to conventional pathology in the assessment of BE. |
| Databáze: | OpenAIRE |
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