Evaluation of the phenolic profile and immunoreactivity of Mal d 3 allergen in ancient apple cultivars from Italy
| Autor: | Gabriella Pasini, Matteo Marangon, Barbara Simonato, Mara Vegro, Simone Vincenzi |
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| Rok vydání: | 2019 |
| Předmět: |
030309 nutrition & dietetics
apple ancient cultivar Biology medicine.disease_cause phenolic profile Antioxidants 03 medical and health sciences 0404 agricultural biotechnology Allergen Phenols medicine Cultivar 0303 health sciences Nutrition and Dietetics fungi food and beverages Hypoallergenic 04 agricultural and veterinary sciences Antigens Plant 040401 food science Mal d 3 allergen Northern italy Horticulture Italy Fruit Malus Agronomy and Crop Science Nutritive Value Food Science Biotechnology |
| Zdroj: | Journal of the science of food and agricultureREFERENCES. 100(13) |
| ISSN: | 1097-0010 |
| Popis: | Background Since the second half of the 20th century, the cultivation of ancient and local apple cultivars has almost disappeared from orchards in Italy. Some of these ancient apple cultivars often possess high nutraceutical values and display lower allergenicity than the modern ones, supporting the so-called 'green revolution' theory. Results In this study, the phenolic composition and the antioxidant activity of five ancient apple cultivars ('Belfiore', 'Pomella Genovese', 'Gravenstein', 'Bella del Bosco', and 'Piatlin') were compared with a 'Golden Delicious' commercial cultivar. Additionally, apples were tested for their potential allergenicity by detecting the presence of Mal d 3, a non-specific lipid transfer protein that represents the main apples' allergen. All apples came from northern Italy (Trentino Region) and were organically produced. Results showed that, for all cultivars, the skins contained more polyphenols than the pulps. 'Bella del Bosco' had the highest amount of polyphenols and antioxidant activity, whereas 'Piatlin' had the lowest phenolic content. All ancient cultivars presented a higher amount of pulp phenolic compounds than 'Golden Delicious'. Immunoblotting techniques showed that 'Bella del Bosco' and 'Piatlin' had very low quantities of Mal d 3 allergen; hence, they can be considered hypoallergenic cultivars. Conclusions The preservation of ancient apple cultivars would be of great importance, not only to maintain the biodiversity but also for their nutritional properties. The hypoallergenic activity of some of these cultivars could be of interest also for the preparation of different apple-based products. © 2020 Society of Chemical Industry. |
| Databáze: | OpenAIRE |
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