The channel kinase, TRPM7 , is required for early embryonic development
| Autor: | Nancy C. Andrews, David E. Clapham, Xiping Cheng, Long Jun Wu, Jie Jin, Haoxing Xu, Janice Jun |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Pluripotent Stem Cells Cellular differentiation Mesenchyme Embryonic Development TRPM Cation Channels Mice Transgenic Nerve Tissue Proteins Biology Nestin Mice Intermediate Filament Proteins medicine Animals Induced pluripotent stem cell Multidisciplinary Embryogenesis Neural crest Cell Differentiation Embryonic stem cell Molecular biology medicine.anatomical_structure PNAS Plus Ureteric bud Female |
| Zdroj: | Proceedings of the National Academy of Sciences. 109 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1120033109 |
| Popis: | Global disruption of transient receptor potential-melastatin-like 7 ( Trpm7 ) in mice results in embryonic lethality before embryonic day 7. Using tamoxifen-inducible disruption of Trpm7 and multiple Cre recombinase lines, we show that Trpm7 deletion before and during organogenesis results in severe tissue-specific developmental defects. We find that Trpm7 is essential for kidney development from metanephric mesenchyme but not ureteric bud. Disruption of neural crest Trpm7 at early stages results in loss of pigment cells and dorsal root ganglion neurons. In contrast, late disruption of brain-specific Trpm7 after embryonic day 10.5 does not alter normal brain development. We developed induced pluripotent stem cells and neural stem (NS) cells in which Trpm7 disruption could be induced. Trpm7 −/− NS cells retained the capacities of self-renewal and differentiation into neurons and astrocytes. During in vitro differentiation of induced pluripotent stem cells to NS cells, Trpm7 disruption prevents the formation of the NS cell monolayer. The in vivo and in vitro results demonstrate a temporal requirement for the Trpm7 channel kinase during embryogenesis. |
| Databáze: | OpenAIRE |
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