The A+T-rich sequence of the simian virus 40 origin is essential for replication and is involved in bending of the viral DNA
| Autor: | G Z Hertz, M R Young, J E Mertz |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
DNA Replication
Genes Viral Base pair Immunology Mutant Simian virus 40 Virus Replication medicine.disease_cause Microbiology Restriction fragment chemistry.chemical_compound Virology medicine Promoter Regions Genetic Base Composition Mutation COS cells Base Sequence biology Nucleic acid sequence Molecular biology Thymine chemistry Insect Science DNA Viral biology.protein Nucleic Acid Conformation DNA Research Article |
| Zdroj: | Journal of Virology. 61:2322-2325 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.61.7.2322-2325.1987 |
| Popis: | The origin-promoter region of simian virus 40 contains a 17-base-pair sequence composed exclusively of adenine (A) and thymine (T). We constructed a linker replacement mutant in which this stretch of A's and T's was reduced to 11 base pairs. While not affecting the level of early gene transcription, this mutation reduced the accumulation of viral DNA in COS cells at least 10(4) fold. In addition, a restriction fragment containing the wild-type A + T-rich region migrated in nondenaturing polyacrylamide gels with an anomalous mobility characteristic of bent DNA; however, the corresponding fragment from the mutant migrated less anomalously. Therefore, bending of the DNA in this region may play a role in some step in viral DNA replication. |
| Databáze: | OpenAIRE |
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