Characterization of LIC11207, a novel leptospiral protein that is recognized by human convalescent sera and prevents apoptosis of polymorphonuclear leukocytes
| Autor: | Marina V. Atzingen, Gabriela Pretre, Ricardo Martín Gómez, Mirta Schattner, Ana L. T. O. Nascimento, Maria Jose Lapponi |
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| Rok vydání: | 2013 |
| Předmět: |
Antigenicity
Neutrophils Virulence Factors Otras Ciencias Biológicas Hamster Apoptosis Biology medicine.disease_cause Microbiology law.invention Ciencias Biológicas law Cricetinae medicine Animals Humans Leptospirosis Escherichia coli Gene Antigens Bacterial Expression vector Gene Expression Profiling Endothelial Cells biology.organism_classification Antibodies Bacterial Molecular biology LEPTOSPIRA INTERROGANS Disease Models Animal Kidney Tubules Infectious Diseases Recombinant DNA Leptospira interrogans RECOMBINANT PROTEIN LEPTOSPIROSIS CIENCIAS NATURALES Y EXACTAS Bacterial Outer Membrane Proteins |
| Zdroj: | Microbial Pathogenesis. 56:21-28 |
| ISSN: | 0882-4010 |
| Popis: | We report the study of a predicted outer-membrane leptospiral protein encoded by the gene lic11207. This protein is conserved in several pathogenic leptospiral strains but is absent in the saprophyte Leptospira biflexa. This putative outer-membrane protein has a domain of unknown function (DUF) 1565 found in several phylogenetically diverse bacteria and in the archaeon Methanosarcina acetivorans. The gene was cloned and expressed in Escherichia coli BL21 (SI) strain using the expression vector pDEST17. The 34 kDa recombinant protein was tagged with N-terminal hexahistidine and purified by metal-charged chromatography. The purified protein was used to assess: reactivity with human convalescent sera; in vivo expression; ability to activate endothelial cells (EC); and ability to modulate the apoptosis of polymorphonuclear cells (PMNs). The LIC11207 coding sequence was identified in vivo in the hamster renal tubules during experimental infection with Leptospira interrogans. The rLIC11207 showed significant antigenicity against human convalescent sera when compared with sera from healthy donors. The recombinant protein did not alter the surface expression of E-selectin or intercellular adhesion molecule 1 (ICAM-1) in EC and failed to induce the release of von Willebrand factor (vWF). Interestingly, rLIC11207 delayed apoptosis of PMNs suggesting a possible role of this protein during the infection. © 2012 Elsevier Ltd. Fil: Prêtre, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Lapponi, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina Fil: Atzingen, Marina V.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil Fil: Schattner, Mirta Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina Fil: Nascimento, Ana L.T.O.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil. Universidade de Sao Paulo; Brasil Fil: Gomez, Ricardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina |
| Databáze: | OpenAIRE |
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