Manipulation of the helper T cell response to influence antigenic competition occurring with a multivalent vaccine
| Autor: | Paul R. Wood, Jacqueline D. Hunt, David C. Jackson, Lorena E. Brown, David J. Stewart |
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| Rok vydání: | 1996 |
| Předmět: |
0301 basic medicine
T cell Molecular Sequence Data Immunology Fimbria Antigen presentation Sheep Diseases Priming (immunology) Epitope Microbiology Mice 03 medical and health sciences 0302 clinical medicine Bacterial Proteins Antigen medicine Animals Immunology and Allergy Cytotoxic T cell Amino Acid Sequence Foot Rot Antigen Presentation Immunity Cellular Mice Inbred BALB C Vaccines Synthetic Gram-Negative Anaerobic Bacteria Sheep biology Vaccination T-Lymphocytes Helper-Inducer Cell Biology Virology 030104 developmental biology medicine.anatomical_structure Fimbriae Bacterial biology.protein Antibody Cell Division 030215 immunology |
| Zdroj: | Immunology and Cell Biology. 74:81-89 |
| ISSN: | 0818-9641 |
| DOI: | 10.1038/icb.1996.11 |
| Popis: | The reduction in antibody observed following inoculation with multiple heterologous Dichelobacter nodosus pili antigens is thought to be due to competition between antigen-specific B cells for a limited amount of T cell help. We demonstrate here that this competition is not further influenced by the expansion of cross-reactive antibody secreting cells at the expense of serogroup specific antibody secreting cells. The T cell determinants of pili recognized by sheep and BALB/c mice have been defined using 15 residue peptides. These T cell determinants include cross-reactive determinants in the conserved amino terminal region of the antigen. Here we investigate the effect of expanding the pili-specific T cell population by priming with pili derived T cell determinants. It was not possible to increase the antibody elicited in response to the multivalent vaccine by priming mice with either a synthetic peptide spanning a T cell determinant or with reduced and alkylated or heterologous serogroups of pili 4 weeks before inoculation with the multivalent vaccine. A strategy designed to increase the T cell population by inoculating animals with pili covalently coupled to an extrinsic T cell determinant was pursued. |
| Databáze: | OpenAIRE |
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