Physicochemical characterization and cytotoxicity of articaine-2-hydroxypropyl-β-cyclodextrin inclusion complex

Autor: Francisco Carlos Groppo, Luis Fernando Cabeça, Eneida de Paula, Jonny Burga-Sánchez, Maria Cristina Volpato, Mário Antônio Braga, Luiz Eduardo Nunes Ferreira, Leonardo Fernandes Fraceto
Přispěvatelé: Universidade Estadual de Campinas (UNICAMP), Guarulhos University, Parana Federal Technological University – Londrina City, Universidade Estadual Paulista (Unesp)
Rok vydání: 2020
Předmět:
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 1432-1912
0028-1298
Popis: Made available in DSpace on 2020-12-12T02:07:07Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-07-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Articaine (ATC) is one of the most widely used local anesthetics in dentistry. Despite its safety, local toxicity has been reported. This study aimed to develop an ATC-2- hydroxypropyl-β-cyclodextrin inclusion complex (ATC HPβCD) and to assess its toxicity in vitro. The inclusion complex was performed by solubilization, followed by a fluorimetric and job plot assay to determine the complex stoichiometry. Scanning electron microscopy, DOSY- 1 H-NMR, differential scanning calorimetry (DSC), and sustained release kinetics were used to confirm the inclusion complex formation. In vitro cytotoxicity was analyzed by MTT assay and immunofluorescence in HGF cells. Fluorimetric and job plot assay determined the inclusion complex stoichiometry (ATC:HPβCD = 1:1) and complex formation time (400 min), as indicated by a strong host/guest interaction (Ka = 117.8 M − 1), complexed fraction (f = 41.4%), and different ATC and ATC HPβCD melting points (172 °C e 235 °C, respectively). The mean of cell viability was 31.87% and 63.17% for 20-mM ATC and 20-mM ATC HPβCD, respectively. Moreover, remarkable cell toxicity was observed with free ATC by immunofluorescence. These results indicate the ATC HPβCD complex could be used to improve the safety of ATC. Further research are needed to establish the anesthetic safety and effectiveness in vivo. Department of Physiological Sciences Piracicaba Dental School University of Campinas – UNICAMP, Avenida Limeira, 901 Laboratory of Inflammation and Immunology Guarulhos University, Praça Teresa Cristina, 229 - Centro Parana Federal Technological University – Londrina City, Avenida dos Pioneiros 3131, Jd Morumbi Department of Biochemistry Biology Institute University of Campinas – UNICAMP, Rua Monteiro Lobato, 255 Laboratory of Environmental Nanotechnology Institute of Science and Technology of Sorocaba São Paulo State University (UNESP), Av. Três de Março, 511 - Aparecidinha Laboratory of Environmental Nanotechnology Institute of Science and Technology of Sorocaba São Paulo State University (UNESP), Av. Três de Março, 511 - Aparecidinha FAPESP: #2015/20942-6
Databáze: OpenAIRE
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