Antitumor effect of whole body hyperthermia with alpha-galactosylceramide in a subcutaneous tumor model of colon cancer
| Autor: | Satoshi Kokura, Takeshi Hattori, Osamu Handa, Norimasa Yoshida, Toshikazu Yoshikawa, Tomohisa Takagi, Tetsuya Okayama, Yuji Naito, Toshimitsu Okuda |
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| Rok vydání: | 2007 |
| Předmět: |
Hyperthermia
Male Cancer Research Pathology medicine.medical_specialty Physiology Colorectal cancer medicine.medical_treatment chemical and pharmacologic phenomena Galactosylceramides Interferon-gamma Mice Immune system Antigen Physiology (medical) Intensive care Cell Line Tumor medicine Animals Humans Chemotherapy Mice Inbred BALB C biology business.industry Cancer Hyperthermia Induced medicine.disease Combined Modality Therapy carbohydrates (lipids) CD1D Colonic Neoplasms Cancer research biology.protein lipids (amino acids peptides and proteins) business T-Lymphocytes Cytotoxic |
| Zdroj: | International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group. 23(7) |
| ISSN: | 0265-6736 |
| Popis: | Whole body hyperthermia (WBH) has been used clinically as an adjunct to radio- and chemotherapy in patients with various cancers. Recently, it has been reported that an activation of the immune system has recently been reported as a possible contributor to the therapeutic effects of WBH. Conversely, the glycolipid alpha-galactosylceramide (alpha-GalCer) is recognized by natural killer (NK) T cells together with the monomorphic MHC-like antigen, CD1d, in mice and humans. This study investigated the antitumor effects of WBH combined with alpha-GalCer in a mouse subcutaneous tumor model of colon cancer.Colon26 cells were inoculated subcutaneously into male BALB/c mice to establish subcutaneous tumor. Colon26-bearing mice were treated with WBH using far infrared rays three times/week. Rectal temperature was maintained for 60 min at 41 degrees C. In some experimental groups, alpha-GalCer was intraperitoneally injected before WBH. We investigated the therapeutic effects of WBH, alpha-GalCer and combined therapy.(1) Compared with controls, WBH alone resulted in significant inhibition of tumor growth. (2) No inhibitory effect on tumor growth was seen with alpha-GalCer. (3) The combination of WBH and alpha-GalCer showed significant inhibition of tumor growth and prolongation of survival. (4) Serum IFN-gamma increased after 3 h and returned to basal levels by 24 h after alpha-GalCer administration. (5) CTL activity was enhanced following combination therapy with WBH and alpha-GalCer.WBH showed antitumor effects in a mouse subcutaneous tumor model of colon cancer. Addition of alpha-GalCer increased the efficacy of WBH, probably via enhancement of immune response. |
| Databáze: | OpenAIRE |
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