Association assessment of Interleukine-10 gene polymorphism and its expression status with susceptibility to coronary artery disease in Iran
| Autor: | Eisa Jorjani, Touraj Farazmandfar, Reza Salehi Manzari, Majid Shahbazi, Aref Salehi, Seyedeh Zahra Mousavi |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
lcsh:QH426-470 medicine.medical_treatment Disease Biology Coronary artery disease 03 medical and health sciences 0302 clinical medicine Genotype medicine Polymorphism Allele Genetics (clinical) lcsh:R5-920 Promoter medicine.disease Interleukin-10 SSP-PCR lcsh:Genetics Interleukin 10 030104 developmental biology Cytokine 030220 oncology & carcinogenesis Immunology Gene polymorphism lcsh:Medicine (General) |
| Zdroj: | Egyptian Journal of Medical Human Genetics; Vol 19, No 1 (2018); 31–35 Egyptian Journal of Medical Human Genetics, Vol 19, Iss 1, Pp 31-35 (2018) |
| ISSN: | 1110-8630 |
| DOI: | 10.1016/j.ejmhg.2017.06.005 |
| Popis: | Background The cytokines are potent inflammatory factors that regulate each stage of atherosclerosis leading to the disease development. Interleukin-10 (IL-10) as an anti-inflammatory cytokine can develop atherosclerosis by inhibiting the synthesis of metalloproteinase. Moreover, IL-10 promotes the plaque stability by preserving the extracellular matrix and fibrous cap. Aim We evaluated the association of the two IL-10 promoter gene polymorphisms with susceptibility to coronary artery disease (CAD) in Iranian population. Subjects and methods We used the Sequence Specific Primer-Polymerase Chain Reaction method to determine genotypes. We also studied mRNA expression of the IL-10 gene in Iranian CAD patients using quantitative real-time PCR. Results There was a significant association between IL-10(−819) T allele and IL-10(−819) T/T genotype, and CAD (p = 0.041 and p = 0.042 respectively). There also was a significant association between IL-10(−1082) G allele and IL-10(−1082) G/G genotype, and CAD (p = 0.017 and p = 0.020 respectively). Genotype T/T of IL-10(−819) polymorphism significantly associated with the two vessel disease type (p = 0.017). In addition, there was a significant association between IL-10(−1082) G/G genotype and the three vessel disease type (p = 0.009). IL-10 mRNA expression was significantly decreased 3.36-fold in samples with IL-10(−819) polymorphism and 1.98-fold in individuals with IL-10(−1082) polymorphism. Conclusions Our results suggest that IL-10 gene promoter polymorphisms may influence both coronary artery disease risks and severity in Iranian patients. |
| Databáze: | OpenAIRE |
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