| Autor: |
Pérez-Pérez, W. D., Carrasco-Navarro, U., García‑Estrada, C., Kosalková, K., Gutiérrez-Ruíz, M. C., Barrios-González, J., Fierro, F. |
| Rok vydání: |
2022 |
| DOI: |
10.6084/m9.figshare.19500854 |
| Popis: |
Additional file 1. Strategy for silencing of expression of Pc-yap1 and Pc-rsmA. (A) A DNA fragment of 458 bp from the exon 2 of Pc-yap1 was amplified by PCR with primers siYAP1-F and -R, digested with NcoI and inserted at the NcoI site of plasmid pGpdPki-RNAi to obtain plasmid pGpdPki-RNAi/PcYap1. (B) A DNA fragment of 409 bp from the exon 3 of the Pc-rsmA gene was amplified by PCR with primers siRsmA-F and -R, digested with NcoI and inserted at the NcoI site of plasmid pGpdPki-RNAi to obtain plasmid pGpdPki-RNAi/PcRsmA. (C) Strategy of silencing. Transcription from the opposite-oriented promoters gpd and pki generates complementary RNA strands that form a dsRNA with the sequence of the inserted 458 bp fragment from Pc-yap1. This dsRNA will cause silencing of the expression of Pc-yap1 through the RNAi pathway. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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