Serum IgA1 shows increased levels of α2,6-linked sialic acid in breast cancer
| Autor: | Aristotelis Antonopoulos, Stuart M. Haslam, Hannah J. Lomax-Browne, Miriam Dwek, Claire E. Robertson, Anthony J. C. Leathem, Anne Dell |
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| Přispěvatelé: | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Glycan
Glycosylation glycosylation Biomedical Engineering Biophysics Bioengineering chemical and pharmacologic phenomena Biochemistry Biomaterials 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Breast cancer breast cancer fluids and secretions Antigen stomatognathic system medicine skin and connective tissue diseases 030304 developmental biology 0303 health sciences biology Cancer Articles medicine.disease Molecular biology Sialic acid chemistry 030220 oncology & carcinogenesis biology.protein Biomarker (medicine) Immunohistochemistry lectin ELISA IgA Biotechnology |
| Popis: | The lectinHelix pomatiaagglutinin (HPA) recognizes altered glycosylation in solid cancers and the identification of HPA binding partners in tumour tissue and serum is an important aim. Among the many HPA binding proteins, IgA1 has been reported to be the most abundant in liver metastases. In this study, the glycosylation of IgA1 was evaluated using serum samples from patients with breast cancer (BCa) and the utility of IgA1 glycosylation as a biomarker was assessed. Detailed mass spectrometric structural analysis showed an increase in disialo-biantennaryN-linked glycans on IgA1 from BCa patients (p< 0.0001: non-core fucosylated;p= 0.0345: core fucosylated) and increased asialo-Thomsen–Friedenreich antigen (TF) and disialo-TF antigens in theO-linked glycan preparations from IgA1 of cancer patients compared with healthy control individuals. An increase inSambucus nigrabinding was observed, suggestive of increasedα2,6-linked sialic acid on IgA1 in BCa. Logistic regression analysis showed HPA binding to IgA1 and tumour size to be significant independent predictors of distant metastases (χ213.359;n= 114;p= 0.020) with positive and negative predictive values of 65.7% and 64.6%, respectively. Immunohistochemical analysis of tumour tissue samples showed IgA1 to be detectable in BCa tissue. This report provides a detailed analysis of serum IgA1 glycosylation in BCa and illustrates the potential utility of IgA1 glycosylation as a biomarker for BCa prognostication. |
| Databáze: | OpenAIRE |
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