Effects of immunization against α-inhibin using two adjuvants on daily sperm production and hormone concentrations in ram lambs
| Autor: | J.E. Wheaton, J.L. Voge, J.B. Parker |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
endocrine system medicine.medical_specialty medicine.drug_class medicine.medical_treatment Freund's Adjuvant Biology Immunostimulant Follicle-stimulating hormone Endocrinology Adjuvants Immunologic stomatognathic system Food Animals Internal medicine Testis Blood plasma medicine Animals Inhibins Testosterone Spermatogenesis Sheep Sperm Count Organ Size Luteinizing Hormone Seminiferous Tubules Antibodies Neutralizing Titer Immunization Animal Science and Zoology Follicle Stimulating Hormone Luteinizing hormone Adjuvant |
| Zdroj: | Domestic Animal Endocrinology. 37:206-213 |
| ISSN: | 0739-7240 |
| DOI: | 10.1016/j.domaniend.2009.05.005 |
| Popis: | Twenty-five ram lambs were immunized against alpha-inhibin peptide emulsified in Freund's adjuvant (FRA), Emulsigen (EML) containing an oligodeoxynucleotide as an immunostimulant, or adjuvant without alpha-inhibin antigen (control). Four immunizations were administered during an 85-d period, after which testes were obtained for determination of daily sperm production (DSP) and histological evaluation. alpha-Inhibin antibody (Ab) titers were 70-fold greater in lambs treated with FRA than in EML-treated ram lambs. alpha-Inhibin immunization had no effect on testes weight or on plasma concentrations of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone. Mean DSP/g tended (P=0.1) to be greater in alpha-inhibin-immunized (EML=17.6x10(6); FRA=15.8x10(6)) ram lambs than in control animals (14.4x10(6)). One of the 8 control ram lambs had an elevated DSP/g, which was a statistical outlier. Without data from this lamb, DSP/g was increased (P0.01) in alpha-inhibin-immunized ram lambs by 28% over controls. No association was found between the titer of alpha-inhibin Ab developed and DSP/g. Histologically, the percentage of testicular area occupied by seminiferous tubules differed (P=0.01) by treatment and was greatest (82%) in EML-treated ram alpha-inhibin-immunized lambs and lowest (74%) in control animals. Percentage tubular area and DSP/g were correlated (r=0.57, P=0.003). Findings show that (1) the extent of the increase in DSP/g is not dependent on the titer of alpha-inhibin Ab; (2) the increase in DSP/g is achieved through an increase in the mass of seminiferous tubules; and (3) FRA elicits a greater alpha-inhibin Ab titer than EML containing an oligodeoxynucleotide. |
| Databáze: | OpenAIRE |
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