Simplified method for conjugating macrocyclic bifunctional chelating agents to antibodies via 2-iminothiolane
| Autor: | Habibe Diril, Michael J. McCall, Claude F. Meares |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
medicine.drug_class
Antibody Affinity Biomedical Engineering Pharmaceutical Science Bioengineering Monoclonal antibody chemistry.chemical_compound Heterocyclic Compounds Imidoesters medicine Chelation Cobalt Radioisotopes 2-Iminothiolane Bifunctional Chromatography High Pressure Liquid Chelating Agents Pharmacology biology Organic Chemistry Antibodies Monoclonal Combinatorial chemistry Cross-Linking Reagents chemistry Reagent Posttranslational modification biology.protein Amine gas treating Antibody Biotechnology |
| Zdroj: | Bioconjugate Chemistry. 1:222-226 |
| ISSN: | 1520-4812 1043-1802 |
| DOI: | 10.1021/bc00003a007 |
| Popis: | A one-step method for conjugating macrocyclic chelators to antibodies using the protein modification reagent 2-iminothiolane controls aggregation, maintains immunoreactivity, and produces consistent chelate/antibody ratios. Conjugation conditions have been investigated with the macrocyclic chelates 6-[p-(bromoacetamido)benzyl]-1,4,8,11-tetraazacyclotetradecane-N,N ',N",N"'-tetraacetic acid and 2-[p-(bromoacetamido)benzyl]-1,4,7,10-tetraazacyclododecane-N,N',N ",N"'-tetraacetic acid, with three different monoclonal antibodies. The bifunctional chelating agents are prepared by bromoacetylation of their amine precursors using a two-phase H2O/CHCl3 system, which improves product purity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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