Endothelin 3 induces the reversion of melanocytes to glia through a neural crest-derived glial-melanocytic progenitor
| Autor: | Pierre Vaigot, Corinne Glavieux, Elisabeth Dupin, Nicole M. Le Douarin |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Cellular differentiation
Cell Separation Melanocyte Biology Quail Culture Techniques medicine Animals education Endothelin-3 education.field_of_study Multidisciplinary Epidermis (botany) Stem Cells Neural crest Cell Differentiation Biological Sciences Embryonic stem cell Clone Cells Cell biology Endothelin 3 medicine.anatomical_structure Neural Crest Immunology Melanocytes Neuroglia Epidermis Mitogens Stem cell |
| Zdroj: | Proceedings of the National Academy of Sciences. 97:7882-7887 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Functional signaling of endothelin 3 (ET3) and its receptor B (ETRB) has been shown to be required for the development of neural crest (NC)-derived pigment cells in mouse, but the precise role of ET3 is not completely understood. Using the avian embryo as a model, we previously reported that ET3 promotes the survival and proliferation of unipotent melanocyte and bipotent glia-melanocyte precursors in trunk NC cultures. Here we investigated whether, at later stages, embryonic pigment cells respond to ET3. Such a possibility is supported by the previous finding that, in vivo , avian melanocytes express endothelin receptor B2 (ETRB2) during migration and after their differentiation in the skin. We found that in vitro ET3 exerts a dose-dependent stimulation of proliferation and melanogenesis in NC cells that had homed to the epidermis of embryonic quail dorsal skin. Moreover, in clonal cultures of skin-derived pigment cells, ET3 induces rapid cell divisions of clonogenic melanocytes that generate a mixed progeny of melanocytes and cells devoid of pigment granules and expressing glial markers in more than 40% of the colonies. It can therefore be concluded that ET3 is strongly mitogenic to embryonic pigment cells and able to alter their differentiation program, leading them to recapitulate the glial-melanocyte bipotentiality of their NC ancestors. |
| Databáze: | OpenAIRE |
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