Involvement of CD40 Targeting miR-224 and miR-486 on the Progression of Pancreatic Ductal Adenocarcinomas
| Autor: | Christina Schleicher, Edith Willscher, Norbert Senninger, Soeren Torge Mees, Joerg Haier, Wolf Arif Mardin, Mario Colombo-Benkmann, Sonja Sielker |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Pathology medicine.medical_specialty endocrine system diseases Microarray Cell Mice Nude Adenocarcinoma Biology Flow cytometry Metastasis Mice Downregulation and upregulation Surgical oncology microRNA Tumor Cells Cultured medicine Animals Humans RNA Messenger Epigenetics CD40 Antigens Oligonucleotide Array Sequence Analysis medicine.diagnostic_test Gene Expression Profiling Flow Cytometry medicine.disease Xenograft Model Antitumor Assays digestive system diseases Gene Expression Regulation Neoplastic Pancreatic Neoplasms MicroRNAs medicine.anatomical_structure Oncology Disease Progression Surgery Carcinoma Pancreatic Ductal |
| Zdroj: | Annals of Surgical Oncology. 16:2339-2350 |
| ISSN: | 1534-4681 1068-9265 |
| DOI: | 10.1245/s10434-009-0531-4 |
| Popis: | Genetic and epigenetic alterations during development of pancreatic ductal adenocarcinomas (PDAC) are well known. Genetic and epigenetic data were correlated with tumor biology to find specific alterations responsible for invasion and metastasis in pancreatic ductal adenocarcinomas. A total of 16 human PDAC cell lines were used in murine orthotopic PDAC models. By means of standardized dissemination scores, local invasion and metastatic spread were assessed. mRNA and microRNA expression were studied by microarray and TaqMan low-density array. Quantitative real-time–polymerase chain reaction and flow cytometry were used for expression validation. CD40 was detected as a relevant target gene for differentially expressed miRNAs observed in highly invasive and metastatic PDAC only. A significant overexpression (P |
| Databáze: | OpenAIRE |
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