Cytological and molecular diagnosis of Hürthle cell thyroid tumors: Analysis of three cases
| Autor: | Yulia A Veryaskina, Vladimir V Anishchenko, Tatiana L Poloz, S. E. Titov |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Hürthle cell mitochondrial DNA Malignancy Hurthle Cell Tumor Thyroid carcinoma 03 medical and health sciences 0302 clinical medicine medicine Atypia thyroid gland microRNA business.industry Thyroid Cancer Articles Cell cycle medicine.disease Molecular medicine medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis fine-needle biopsy 030211 gastroenterology & hepatology business |
| Zdroj: | Molecular and Clinical Oncology |
| ISSN: | 2049-9469 2049-9450 |
| Popis: | The cytological diagnosis of Hürthle cell (oncocytic) thyroid tumors by means of fine-needle aspiration biopsy represents a challenge, as Hürthle cell polymorphism and atypia alone are not indications of malignancy. In our recent work, an original diagnostic algorithm was proposed, which identified and typed malignant thyroid tumors by analyzing the molecular markers of cytological preparations. The aim of the present study was to assess the effectiveness of this algorithm at detecting Hürthle cell thyroid tumors in clinical samples used for cytological examination. Cytological and histological examinations of the biopsy material were performed for three patients with nodular neoplasms. Biopsy material of these patients was analyzed by quantitative PCR using preselected molecular markers [normalized concentrations of High-mobility group AT-hook 2 mRNA, three microRNAs (miRNAs or miRs; miR-146b, miR-221 and miR-375) and the mitochondrial (mtDNA)/nuclear DNA ratio]. The results revealed that the molecular test determined the malignancy of three cases of Hürthle cell tumor. This method may therefore be used to complement the cytological diagnosis of fine-needle aspiration biopsy. In all three cases, there was an increased content of mtDNA, indicating Hürthle cell malignancies. Furthermore, in the first case [Hürthle cell carcinoma (HCC)], increased miRNA-221 content was detected, which also indicated malignancy. In the second case (Hürthle cell papillary thyroid carcinoma), an increased level of miRNA-146b was present, which indicated papillary carcinoma. In the third case (Hürthle cell adenoma), no markers of malignancy were identified. The present study demonstrated that molecular testing together with cytological analysis can reduce the isk of error in the preoperative cytological diagnosis of unclear or ambivalent cases. |
| Databáze: | OpenAIRE |
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