Spherical Nanoparticle Supported Lipid Bilayers for the Structural Study of Membrane Geometry-Sensitive Molecules
| Autor: | Jie Xu, Nicole E. Briley, Conggang Li, Erin R. Tyndall, Richard L. Gill, Fang Tian, John M. Flanagan, Kumaran S. Ramamurthi, Edward Y. Kim, Riqiang Fu |
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| Rok vydání: | 2015 |
| Předmět: |
Magnetic Resonance Spectroscopy
Molecular Structure Chemistry Vesicle Cell Membrane Lipid Bilayers Membrane Proteins Geometry Biological membrane General Chemistry Membrane transport Biochemistry Article Catalysis Exocytosis Colloid and Surface Chemistry Membrane Bacterial Proteins Membrane curvature Membrane fluidity Nanoparticles Lipid bilayer |
| Zdroj: | Journal of the American Chemical Society. 137:14031-14034 |
| ISSN: | 1520-5126 0002-7863 |
| Popis: | Many essential cellular processes including endocytosis and vesicle trafficking require alteration of membrane geometry. These changes are usually mediated by proteins that can sense and/or induce membrane curvature. Using spherical nanoparticle supported lipid bilayers (SSLBs), we characterize how SpoVM, a bacterial development factor, interacts with differently curved membranes by magic angle spinning solid-state NMR. Our results demonstrate that SSLBs are an effective system for structural and topological studies of membrane geometry-sensitive molecules. |
| Databáze: | OpenAIRE |
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