Introgression of drought tolerance root traits into Kenyan commercial chickpea varieties using marker assisted backcrossing

Autor:	A.J. Kosgei, P.K. Kimurto, P.M. Gaur, M.A. Yeboah, S.K. Offei, E.Y. Danquah
Jazyk:	angličtina
Rok vydání:	2022
Předmět:	Polymorphic markers quantitative trait loci terminal drought food and beverages General Medicine
Zdroj:	African Crop Science Journal; Vol. 30 No. 1 (2022); 31-50
ISSN:	1021-9730 2072-6589
Popis:	Roots play critical roles in enhancing drought tolerance, more so under terminal drought conditions. The objective of this study was to introgress drought tolerant root traits into Kenyan chickpea varieties through marker assisted backcrossing (MABC). Eight simple sequence repeat (SSR) markers, linked to quantitative trait loci (QTL) for root traits, were used to screen parents at ICRISAT in India, and 1144 single nucleotide polymorphic (SNPs) markers at Legume Genomics Centre in the United Kingdom. Crosses were made between two selected varieties, ICCV 92944 (Chania Desi II) and ICCV 00108 (LDT 068); and ICC 4958, QTL donor parent. Polymorphic SSR and SNP markers were used to select offspring with root QTL at F1, BC1F1, and BC2F1, and later advanced to BC2F3. BC2F3 families were evaluated for root traits at Egerton University in Kenya in a pot experiment under rain shelter. The BC2F3 families were significantly (P
Databáze:	OpenAIRE
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