PDL1 expression on monocytes is associated with plasma cytokines in Tuberculosis and HIV

Autor: Meseret Abebe, Rawleigh Howe, Helina Mollalign, Katrin Bauer, Vincent C. Marconi, Wegene Tamene, Liya Wassie, Amha Kebede, Ulrich Sack
Jazyk: angličtina
Rok vydání: 2021
Předmět:
RNA viruses
Bacterial Diseases
Male
Physiology
Gene Expression
HIV Infections
Pathology and Laboratory Medicine
Monocytes
B7-H1 Antigen
White Blood Cells
Medical Conditions
Immunodeficiency Viruses
Animal Cells
Immune Physiology
Medicine and Health Sciences
Innate Immune System
Multidisciplinary
Coinfection
virus diseases
Viral Load
Body Fluids
Interleukin 10
medicine.anatomical_structure
Infectious Diseases
Blood
Medical Microbiology
Viral Pathogens
Viruses
Medicine
Cytokines
Tumor necrosis factor alpha
Female
Cellular Types
Pathogens
Anatomy
Viral load
Research Article
Adult
Tuberculosis
CD14
Science
Immune Cells
Immunology
CD16
Microbiology
Blood Plasma
Virology
Retroviruses
medicine
Genetics
Humans
Microbial Pathogens
Lipoarabinomannan
Blood Cells
business.industry
Monocyte
Lentivirus
Organisms
Biology and Life Sciences
HIV
Cell Biology
Molecular Development
medicine.disease
Tropical Diseases
Immune System
Case-Control Studies
Ethiopia
business
Viral Transmission and Infection
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Vol 16, Iss 10, p e0258122 (2021)
ISSN: 1932-6203
Popis: Introduction PDL1 and its interaction with PD1 is implicated in immune dysfunction in TB and HIV. The expression of PDL1 on multiple subsets of monocytes as well as their associations with cytokines and microbial products have not been well studied. Method HIV (TB-HIV+), TB (TB+HIV-) and TB/HIV co-infected (TB+HIV+) patients as well as apparently healthy controls (TB-HIV-) were recruited. TB and HIV patients were treatment naïve while TB/HIV patients were both ART naïve and experienced but not yet started TB therapy. Monocyte subsets were evaluated for PDL1 expression by flow cytometry; plasma TNFα, IL6, IP10, IFNγ and IL10 were measured by Luminex; and cytokine mRNA from purified monocytes quantitated by qPCR. The association of PDL1 with cytokines, clinical and microbial indices, including HIV viral load, TB smear microscopy and TB urinary lipoarabinomannan (LAM) were assessed. Results Monocyte expression of PDL1 was significantly higher in TB, HIV and TB/HIV co-infected patients compared with healthy controls (p = 0.0001), with the highest levels in TB/HIV co-infected patients. The highest expression of PDL1 was on intermediate (CD14+CD16+) monocytes in all participant groups. PDL1 strongly correlated with HIV viral load in TB/HIV while weakly correlated in HIV. PDL1 levels moderately correlated with plasma TNFα, IL6, IP10, IFNγ and IL10 level in TB subjects whereas weakly correlated with TNFα and IP10 in HIV patients. However, cytokine mRNA from purified monocytes showed no association with either plasma cytokines or monocyte PDL1 expression, implying that if cytokines modulate PDL1, they are likely not originating from circulating monocytes themselves. These results underscore the importance of further characterization of multiple monocyte subsets and their phenotypic and functional differences in different disease states.
Databáze: OpenAIRE
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