Determination of Tetracycline Antibiotics in Beef and Pork Tissues Using Ion-Paired Liquid Chromatography
| Autor: | William A. Moats |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Chlortetracycline
Residue (complex analysis) Chromatography Swine medicine.drug_class Chemistry Tetracycline Antibiotics Tetracycline antibiotics General Chemistry Oxytetracycline Ion pairs Kidney Liver Tetracyclines medicine Animals Cattle Indicators and Reagents Muscle Skeletal General Agricultural and Biological Sciences Chromatography High Pressure Liquid Analysis method Chromatography Liquid medicine.drug |
| Zdroj: | Journal of Agricultural and Food Chemistry. 48:2244-2248 |
| ISSN: | 1520-5118 0021-8561 |
| Popis: | A simplified procedure was developed for determination of tetracycline antibiotics in tissues which improved stability of these compounds in sample extracts and eliminated the need for troublesome cleanup procedures. Tissues were homogenized in water. Acetonitrile (16 mL) and then 1 mL of 0.1 M H(3)PO(4) were added to 4 mL of homogenate and the clear supernatant was filtered. The filtrate was mixed with hexane and dichloromethane and the resulting water layer was collected, evaporated to 1-2 mL, and filtered into autosampler vials. Ion-pairing liquid chromatography was used to separate tetracyclines from interferences in sample extracts, eliminating the need for further cleanup. Analysis was isocratic using a Phenomonex Prodigy ODS(3) column with a mobile phase of 4 mM oxalic acid, 4 mM sodium oxalate, 10 mM sodium decanesulfonate-acetonitrile (70 + 30 for oxytetracycline and tetracycline; 66 + 34 for chlortetracycline). Recoveries were generally in the 90-100% range with limits of quantitation of 0. 05-0.1 ppm. The procedure was evaluated with beef and pork muscle, liver, and kidney. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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