Simultaneous detection and identification of enteric viruses by PCR-mass assay
| Autor: | Xiaoping Kang, Guan Yanfang, Xueyong Huang, Licheng Liu, Yuling Zheng, Pengzhi Wang, Yuanyin Cao, Hua Jiang, Bianli Xu, Yinhui Yang, Yuan Zhang, Chen Weijun, Zhao Jinyin, Piao Jingzi, Yongqiang Jiang, Weili Wu, Yan Li, Xiaohong Wang, Jun Jiang |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Viral Diseases Infectious Disease Control Applied Microbiology lcsh:Medicine Biology medicine.disease_cause Microbiology Hand Foot and Mouth Disease law.invention RNA Virus Infections Hepatitis E virus law Virology Emerging Viral Diseases Microbial Control Multiplex polymerase chain reaction medicine Humans RNA Viruses Multiplex lcsh:Science Microbial Pathogens Polymerase chain reaction Multidisciplinary Foot-and-mouth disease lcsh:R Outbreak RNA medicine.disease Viral Disease Diagnosis Emerging Infectious Diseases Infectious Diseases Enterovirus Infection Medical Microbiology Co-Infections RNA Viral Medicine lcsh:Q Female RNA extraction Multiplex Polymerase Chain Reaction Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 8, p e42251 (2012) |
| ISSN: | 1932-6203 |
| Popis: | Simultaneous detection of enteric viruses that cause similar symptoms (e.g. hand, foot and mouth disease) is essential to the prevention of outbreaks and control of infections. In this study, a novel PCR-Mass assay combining multiplex polymerase chain reaction (PCR) with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was developed and used for simultaneous detection of eight distinct human enteric viruses. Enteric viral isolates and standard viral RNAs were examined to determine the sensitivity and specificity of the PCR-Mass assay. Clinical performance was evaluated with a total of 101 clinical specimens from patients suspected of having hand, foot and mouth disease (HFMD). The results were compared to those of previous analyses using real-time RT-PCR. The identification of specific viruses and clinical specimens shows that the PCR-Mass assay performed as well as or better than standard methods with respect to indicating the presence of multiplex pathogens in a single specimen. |
| Databáze: | OpenAIRE |
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