Molecular Characterization of the Cytochrome b Gene and In Vitro Atovaquone Susceptibility of Plasmodium falciparum Isolates from Kenya
| Autor: | Wallace D. Bulimo, Mabel Imbuga, Benjamin Opot, Fredrick L. Eyase, Jacob D. Johnson, Luicer A. Ingasia, Edwin Kamau, Hoseah M. Akala |
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| Rok vydání: | 2015 |
| Předmět: |
Plasmodium falciparum
Mutant Protozoan Proteins Microbial Sensitivity Tests Biology Antimalarials Polymorphism (computer science) parasitic diseases medicine Pharmacology (medical) Malaria Falciparum Allele Codon Gene Alleles Atovaquone Pharmacology Genetics Polymorphism Genetic Cytochrome b Wild type Cytochromes b DNA Protozoan biology.organism_classification Kenya Drug Combinations Infectious Diseases Proguanil Susceptibility Mutation medicine.drug |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 59:1818-1821 |
| ISSN: | 1098-6596 0066-4804 |
| Popis: | The prevalence of a genetic polymorphism(s) at codon 268 in the cytochrome b gene, which is associated with failure of atovaquone-proguanil treatment, was analyzed in 227 Plasmodium falciparum parasites from western Kenya. The prevalence of the wild-type allele was 63%, and that of the Y268S (denoting a Y-to-S change at position 268) mutant allele was 2%. There were no pure Y268C or Y268N mutant alleles, only mixtures of a mutant allele(s) with the wild type. There was a correlation between parasite 50% inhibitory concentration (IC 50 ) and parasite genetic polymorphism; mutant alleles had higher IC 50 s than the wild type. |
| Databáze: | OpenAIRE |
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