Small RNA sequencing of cryopreserved semen from single bull revealed altered miRNAs and piRNAs expression between High- and Low-motile sperm populations
| Autor: | I. Fojadelli, T.M. Gliozzi, Federica Turri, Emanuele Capra, Paola Cremonesi, Flavia Pizzi, Barbara Lazzari, Alessandra Stella |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine endocrine system Small RNA Piwi-interacting RNA Cryopreserved Semen Cell Separation piRNA Biology Models Biological Deep sequencing Andrology 03 medical and health sciences Genetics medicine Animals Cluster Analysis Sequencing RNA Small Interfering miRNA Cryopreservation urogenital system High-Throughput Nucleotide Sequencing RNA Sperm Molecular biology MicroRNAs 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Sperm Motility RNA Small Untranslated Cattle RNA Interference Percoll Germ cell Signal Transduction Research Article Biotechnology |
| Zdroj: | BMC genomics BMC Genomics.2017, 18:14 (2017): 1–12. doi:10.1186/s12864-016-3394-7 info:cnr-pdr/source/autori:E. Capra, F. Turri, B. Lazzari, P. Cremonesi, T. M. Gliozzi, I. Fojadelli, A. Stella and F. Pizzi/titolo:Small RNA sequencing of cryopreserved semen from single bull revealed altered miRNAs and piRNAs expression between High-and Low-motile sperm populations/doi:10.1186%2Fs12864-016-3394-7/rivista:BMC genomics/anno:2017/pagina_da:1/pagina_a:12/intervallo_pagine:1–12/volume:BMC Genomics.2017, 18:14 BMC Genomics |
| ISSN: | 1471-2164 |
| DOI: | 10.1186/s12864-016-3394-7 |
| Popis: | Background Small RNAs present in bovine ejaculate can be linked to sperm abnormalities and fertility disorders. At present, quality parameters routinely used in semen evaluation are not fully reliable to predict bull fertility. In order to provide additional quality measurements for cryopreserved semen used for breeding, a method based on deep sequencing of sperm microRNA (miRNA) and Piwi-interacting RNA (piRNA) from individual bulls was developed. To validate our method, two populations of spermatozoa isolated from high and low motile fractions separated by Percoll were sequenced, and their small RNAs content characterized. Results Sperm cells from frozen thawed semen samples of 4 bulls were successfully separated in two fractions. We identified 83 miRNAs and 79 putative piRNAs clusters that were differentially expressed in both fractions. Gene pathways targeted by 40 known differentially expressed miRNAs were related to apoptosis. Dysregulation of miR-17-5p, miR-26a-5p, miR-486-5p, miR-122-5p, miR-184 and miR-20a-5p was found to target three pathways (PTEN, PI3K/AKT and STAT). Conclusions Small RNAs sequencing data obtained from single bulls are consistent with previous findings. Specific miRNAs are differentially represented in low versus high motile sperm, suggesting an alteration of cell functions and increased germ cell apoptosis in the low motile fraction. Electronic supplementary material The online version of this article (doi:10.1186/s12864-016-3394-7) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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