Effect of luminal surface structure of decellularized aorta on thrombus formation and cell behavior

Autor: Masako Ohara, Akio Kishida, Toshiya Fujisato, Naoko Nakamura, Mako Kobayashi, Yoshihide Hashimoto, Tsuyoshi Kimura
Rok vydání: 2021
Předmět:
Pathology
Physiology
Swine
Cell
Smooth Muscle Cells
030204 cardiovascular system & hematology
Biochemistry
Epithelium
0302 clinical medicine
Tissue engineering
Animal Cells
Medicine and Health Sciences
Myocyte
Electron Microscopy
Aorta
Microscopy
0303 health sciences
Multidisciplinary
Decellularization
Tissue Scaffolds
Chemistry
Adhesion
Body Fluids
Extracellular Matrix
Denaturation
Blood
medicine.anatomical_structure
Cardiovascular Diseases
Heart Valve Prosthesis
Medicine
Scanning Electron Microscopy
Collagen
Anatomy
Cellular Types
Research Article
medicine.medical_specialty
Imaging Techniques
Science
Myocytes
Smooth Muscle
Muscle Tissue
Research and Analysis Methods
03 medical and health sciences
In vivo
medicine.artery
Fluorescence Imaging
medicine
Surface structure
Animals
Humans
Thrombus
Molecular Biology Techniques
Molecular Biology
030304 developmental biology
Muscle Cells
Tissue Engineering
Biology and Life Sciences
Proteins
Endothelial Cells
Epithelial Cells
Thrombosis
Cell Biology
medicine.disease
Staining
Biological Tissue
Cardiovascular Anatomy
Microscopy
Electron
Scanning
Blood Vessels
Collagens
Biomedical engineering
Zdroj: PLoS ONE
PLoS ONE, Vol 16, Iss 5, p e0246221 (2021)
ISSN: 1932-6203
Popis: Due to an increasing number of cardiovascular diseases, artificial heart valves and blood vessels have been developed. Although cardiovascular applications using decellularized tissue have been studied, the mechanisms of their functionality remain unknown. To determine the important factors for preparing decellularized cardiovascular prostheses that show good in vivo performance, the effects of the luminal surface structure of the decellularized aorta on thrombus formation and cell behavior were investigated. Various luminal surface structures of a decellularized aorta were prepared by heating, drying, and peeling. The luminal surface structure and collagen denaturation were evaluated by immunohistological staining, collagen hybridizing peptide (CHP) staining, and scanning electron microscopy (SEM) analysis. To evaluate the effects of luminal surface structure of decellularized aorta on thrombus formation and cell behavior, blood clotting tests and recellularization of endothelial cells and smooth muscle cells were performed. The results of the blood clotting test showed that the closer the luminal surface structure is to the native aorta, the higher the anti-coagulant property. The results of the cell seeding test suggest that vascular cells recognize the luminal surface structure and regulate adhesion, proliferation, and functional expression accordingly. These results provide important factors for preparing decellularized cardiovascular prostheses and will lead to future developments in decellularized cardiovascular applications.
Databáze: OpenAIRE
Externí odkaz: