Metformin counters both lipolytic/inflammatory agents-decreased hormone sensitive lipase phosphorylation at Ser-554 and -induced lipolysis in human adipocytes

Autor:	Daniel M. Frey, Ulrich Keller, Henryk Zulewski, Elisa Bouillet, Kaethi Dembinski, Katharina Timper, Tanja Radimerski, Jean Grisouard, Mirjam Christ-Crain, Beat Müller, Ralph Peterli
Rok vydání:	2011
Předmět:	Adult Lipopolysaccharides Male medicine.medical_specialty IBMX Lipopolysaccharide Physiology Lipolysis Interleukin-1beta Primary Cell Culture 030209 endocrinology & metabolism Hormone-sensitive lipase Stimulation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Physiology (medical) Internal medicine 1-Methyl-3-isobutylxanthine medicine Adipocytes Serine Humans Hypoglycemic Agents Phosphorylation 030304 developmental biology Aged Inflammation 0303 health sciences Forskolin Tumor Necrosis Factor-alpha Colforsin Isoproterenol food and beverages General Medicine Middle Aged Sterol Esterase Metformin 3. Good health Endocrinology chemistry Female medicine.drug
Zdroj:	Archives of physiology and biochemistry
ISSN:	1744-4160
Popis:	Objective: To study the effects of metformin on lipolysis and hormone sensitive lipase (HSL) phosphorylation in human adipocytes treated with lipolytic and inflammatory agents. Methods: Lipolysis and phosphorylation status of HSL were assessed in subcutaneous pre adipocytes surgically isolated from patients and differentiated into mature adipocytes in vitro. Results: Stimulation for 1?h with forskolin isoproterenol and IBMX or stimulation for 24?h with LPS IL 1ß and TNF a increased lipolysis (p?0.05 vs. basal). The phosphorylation of HSL at Ser 554 was decreased while the Ser 552 phosphorylation was increased. Pre incubation with metformin (24?h 1?mM) inhibited forskolin isoproterenol IBMX LPS IL 1ß and TNF a induced glycerol release and prevented p(Ser554)HSL decrease and p(Ser 552)HSL increase due to lipolytic and inflammatory agents. AMPKa1 is involved in metformin induced HSL phosphorylation at Ser 552. Conclusion: Phosphorylation of HSL at Ser 554 inversely correlates with lipolysis and HSL phosphorylation at Ser552 in human adipocytes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c30046f43de269ba3414f104b51484d https://pubmed.ncbi.nlm.nih.gov/21338323 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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