Cold-plasma modification of oxide surfaces for covalent biomolecule attachment
| Autor: | Ferencz S. Denes, Sorin Manolache, Max G. Lagally, Bradley J. Larson, J.M. Helgren, A.Y. Lau |
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| Rok vydání: | 2005 |
| Předmět: |
Surface Properties
Biomedical Engineering Biophysics Oxide Epoxide chemistry.chemical_compound Biopolymers Adsorption Coated Materials Biocompatible Electrochemistry Organic chemistry Oligonucleotide Array Sequence Analysis chemistry.chemical_classification Chemistry Biomolecule Plasma activation Oxides DNA General Medicine Microarray Analysis Cold Temperature Chemical engineering Covalent bond Reagent Gases Crystallization Wet chemistry Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 21:796-801 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2005.02.005 |
| Popis: | While many processes have been developed to modify the surface of glass and other oxides for biomolecule attachment, they rely primarily upon wet chemistry and are costly and time-consuming. We describe a process that uses a cold plasma and a subsequent in vacuo vapor-phase reaction to terminate a variety of oxide surfaces with epoxide chemical groups. These epoxide groups can react with amine-containing biomolecules, such as proteins and modified oligonucleotides, to form strong covalent linkages between the biomolecules and the treated surface. The use of a plasma activation step followed by an in vacuo vapor-phase reaction allows for the precise control of surface functional groups, rather than the mixture of functionalities normally produced. By maintaining the samples under vacuum throughout the process, adsorption of contaminants is effectively eliminated. This process modifies a range of different oxide surfaces, is fast, consumes a minimal amount of reagents, and produces attachment densities for bound biomolecules that are comparable to or better than commercially available substrates. |
| Databáze: | OpenAIRE |
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