Construction of shuttle expression plasmid and stable expression of foreign gene in mycobacteria and E. coli
| Autor: | Cheng Jizhong, Li Dong, Zhang Da-jun, Liang Juqing, Qian Min, Huangfu Yong-mu |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Kanamycin Resistance
Expression vector Base Sequence Genetic Vectors Molecular Sequence Data Biology Origin of replication Molecular biology law.invention Mycobacterium Plasmid law Management of Technology and Innovation Recombinant DNA BCG Vaccine Escherichia coli bacteria pUC19 Transformation Bacterial Cloning Molecular Gene T-DNA Binary system Plasmids |
| Zdroj: | Journal of Tongji Medical University = Tong ji yi ke da xue xue bao. 15(3) |
| ISSN: | 0257-716X |
| Popis: | By employing the pUC19 as a backbone, the regulatory and signal sequences which encode kanamycin resistance, and mycobacterial plasmid origin of replication (oriM) were cloned into the pUC19. The recombinant E. Coli-mycobacteria shuttle expression plasmid pBCG-8000 was constructed. The pBCG-8000 was able to replicate in both E. Coli and mycobacteria (including BCG) systems, and to confer stable kanamycin resistance upon transformants. The study should facilitate the development of BCG and other mycobacteria into multivalent vaccine vectors. |
| Databáze: | OpenAIRE |
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