Characterization of MXR activity in the sea anemone Bunodosoma cangicum exposed to copper
| Autor: | Juliana Zomer Sandrini, Vanessa Abelaira Anjos, Marta Marques Souza, Cláudia B.R. Martinez |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Physiology Health Toxicology and Mutagenesis Drug Resistance Potassium cyanide chemistry.chemical_element ATP-binding cassette transporter 010501 environmental sciences Sea anemone Toxicology 01 natural sciences Biochemistry 03 medical and health sciences chemistry.chemical_compound Animals Potassium Cyanide Cytotoxicity 0105 earth and related environmental sciences biology Cell Biology General Medicine Calcium Channel Blockers biology.organism_classification Copper Sea Anemones 030104 developmental biology Gene Expression Regulation Verapamil chemistry Xenobiotic Adenosine triphosphate Intracellular |
| Zdroj: | Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 202:12-18 |
| ISSN: | 1532-0456 |
| DOI: | 10.1016/j.cbpc.2017.07.003 |
| Popis: | Transmembrane proteins of the ABC family contribute to a multiple xenobiotic resistance (MXR) phenotype in cells, driving the extrusion of toxic substances. This phenotype promotes a high degree of protection against xenobiotics. The present study provides a better understanding of the MXR activity in the podal disk cells of Bunodosoma cangicum exposed to copper, and further establishes the relationship between protein activity (measured by accumulation of rhodamine-B) and bioaccumulation of copper in these cells. Sea anemone cells were exposed for 24 h to copper (0, 7.8 and 15.6 μg/L) in presence and absence of MXR blocker (verapamil 50 μM). Results indicate that copper exposure increases intracellular metal content when ABC proteins were blocked, causing an increase in cellular death. The present study also verified the relationship between MXR activity, ATP depletion, and general metabolic activity (by MTT). MXR activity decreased in treatment groups exposed to copper concentrations of 15.6 μg/L and 10 mM energy depleting potassium cyanide. Metabolic activity increased in cells exposed to 7.8 μg Cu/L, but 15.6 μg Cu/L was similar to 0 and 7.8 μg/L. The presence of copper decreased the ABC proteins expression. The present study improves the knowledge of MXR in anemone cells and shows that this activity is closely associated with copper extrusion. Also, the copper exposure is able to modify the metabolic state and to lead to cytotoxicity when cells cannot defend themselves. |
| Databáze: | OpenAIRE |
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