Cloning and establishment of a line of rats for high levels of voluntary wheel running
| Autor: | Masaki Morishima-Yamato, Hideki Matoba, Fumiko Hisaoka, Yutaka Nakaya, Sachiko Shinomiya, Nagakatsu Harada, Akira Takahashi |
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| Rok vydání: | 2005 |
| Předmět: |
Blood Glucose
Male medicine.medical_specialty Offspring Cloning Organism Breeding Motor Activity Biology Malate dehydrogenase General Biochemistry Genetics and Molecular Biology Running Insulin resistance Myofibrils Malate Dehydrogenase Internal medicine medicine Animals Rats Wistar Selection Genetic General Pharmacology Toxicology and Pharmaceutics Muscle Skeletal Lactate Dehydrogenases Glucose tolerance test medicine.diagnostic_test Glucose transporter Skeletal muscle General Medicine Metabolism Glucose Tolerance Test medicine.disease Phosphofructokinase activity Pedigree Rats Endocrinology medicine.anatomical_structure Phosphofructokinases Models Animal Female human activities |
| Zdroj: | Life Sciences. 77:551-561 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/j.lfs.2004.10.074 |
| Popis: | We generated an original Wistar line of rats that displayed increased levels of wheel running, which we named SPORTS (Spontaneously-Running-Tokushima-Shikoku). Male SPORTS rats ran voluntarily in a running wheel almost six times longer than male control Wistar rats, established without selection for their running activity. The running phenotype of female SPORTS rats was the same as female control Wistar rats. However, male offspring from the cross-mating between a female SPORTS rat and a male control rat also showed a similar level of hyper-running activity as the original SPORTS line. Compared to control rats, male SPORTS rats had lower levels of mean body weight, abdominal fat and plasma insulin after 4 weeks of running. It is likely that all these beneficial changes observed in the SPORTS rats reflected the increases in glucose disposal we observed in oral glucose tolerance tests carried out on the animals. We also found hyper-running caused a significant increase in skeletal muscle oxidative capacity, measured as the ratio of malate dehydrogenase to phosphofructokinase activity, an index of aerobic metabolism. These results indicate that the SPORTS rat may be a good animal model for determining the mechanisms responsible for up-regulation of running motivation, in addition to investigating changes in nutrient metabolism induced by high intensity exercise. |
| Databáze: | OpenAIRE |
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