Quantitative Immunolocalization of a P29 Protein (GRA7), a New Antigen of Toxoplasma Gondii
| Autor: | Dominique Aubert, Jeff Hunt, Linda E. Chovan, Gregory T. Maine, A. Beorchia, Michael Sheu, Henriette Burlet, A. Bonhomme, Michel Pluot, John Tyner, Lawrence V. Howard, Isabelle Villena, J. M. Pinon, Sue Brojanac |
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| Rok vydání: | 1998 |
| Předmět: |
0301 basic medicine
Histology medicine.drug_class Blotting Western Protozoan Proteins Antigens Protozoan Cytoplasmic Granules Monoclonal antibody 03 medical and health sciences Immunolabeling Antigen Extracellular medicine Animals 030102 biochemistry & molecular biology biology Antibodies Monoclonal Toxoplasma gondii Immunogold labelling biology.organism_classification Immunohistochemistry Cell biology Microscopy Electron 030104 developmental biology Anatomy Dense granule Toxoplasma Intracellular |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 46:1411-1421 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1177/002215549804601210 |
| Popis: | Ultrastructural localization of a P29 protein of Toxoplasma gondii was examined on thin sections by an immunogold technique using a P29 antigen-specific monoclonal antibody (5-241-178). Immunolocalization of the P29 protein in extracellular tachyzoites demonstrated that this antigen was present in the dense granules. Thus, we have identified this P29 antigen as the seventh protein (GRA7) to be localized to the dense granules of T. gondii. P29 immunolocalization in intracellular tachyzoites demonstrated association of this antigen with the parasite membrane complex, tubular elements of the intravacuolar network, and with the parasitophorous vacuolar membrane. Our immunolabeling data suggest trafficking of the P29 (GRA7) antigen from the dense granule via the intravacuolar network to the parasitophorous vacuolar membrane on invasion of the tachyzoite into the host cell. (J Histochem Cytochem 46:1411-1421, 1998) |
| Databáze: | OpenAIRE |
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