Fin and gill biopsies are effective nonlethal samples for detection of viral hemorrhagic septicemia virus genotype IVb
| Autor: | Rodman G. Getchell, Chelsea A. Bellmund, Po Ting Wong, Geoffrey H. Groocock, Paul R. Bowser, Emily R. Cornwell, Katherine L. Hambury |
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| Rok vydání: | 2013 |
| Předmět: |
Gills
endocrine system General Veterinary Genotype medicine.medical_treatment Biopsy Intraperitoneal injection Cyprinidae Spleen Biology biology.organism_classification Virology Sensitivity and Specificity Virus Novirhabdovirus medicine.anatomical_structure Rhabdoviridae Infections medicine Animal Fins Animals Viral hemorrhagic septicemia Pimephales promelas Plaque-forming unit |
| Zdroj: | Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 25(2) |
| ISSN: | 1943-4936 |
| Popis: | Nonlethal sampling is becoming a common method to diagnose fish diseases, especially with the availability of molecular testing. Viral hemorrhagic septicemia virus (VHSV) is a viral pathogen of finfish distributed worldwide. Although VHSV has been known to occur in some parts of the world for decades, a new genotype, IVb, recently emerged in the Laurentian Great Lakes of northeastern North America. Golden shiners ( Notemigonus crysoleucas; Mitchill, 1814) and fathead minnows ( Pimephales promelas; Rafinesque, 1820) were exposed to VHSV-IVb doses between 102 and 106 plaque forming units per fish by intraperitoneal injection at 10°C. Both species experienced significant mortality after exposure, ranging from 38% to 52% in golden shiners and from 35% to 95% in fathead minnows. In golden shiners, a fin or gill sample was somewhat less sensitive at detecting VHSV-IVb by quantitative reverse transcription polymerase chain reaction (qRT-PCR) than a pooled organ sample (consisting of liver, anterior and posterior kidney, spleen, and heart), however the relative sensitivity increased when a fin and gill sample were tested in parallel. In fathead minnows, a fin or gill sample tested alone or in parallel was relatively more sensitive than a pooled organ sample by qRT-PCR. Specificity was 100% for all sample types in both species. The results suggest that fin and gill biopsies are useful tools to test for VHSV in live fish. |
| Databáze: | OpenAIRE |
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