Interleukin 34: a new modulator of human and experimental inflammatory bowel disease
| Autor: | Madeleen Bosma, Gisele Lago Martinez, Stephanie Zwicker, Marco Gerling, Sven Almer, Reuben Clark, Elisabeth A. Boström, Mirjam Majster, Jan Söderman |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
IBD
RA rheumatoid arthritis Ileum Inflammation macrophage S2 Inflammatory bowel disease TNF-α tumour necrosis factor α Mice CSF-1 CD Crohn’s disease medicine CSF-1R CSF-1 receptor Animals Humans DSS dextran sodium sulfate Colitis intestine colon epithelial cells Original Paper IBD inflammatory bowel disease Tumor Necrosis Factor-alpha business.industry Interleukins Macrophages qPCR quantitative real-time PCR NF-kappa B Interleukin CSF-1 colony-stimulating factor 1 General Medicine AD atopic dermatitis Inflammatory Bowel Diseases medicine.disease Ulcerative colitis digestive system diseases IKKβ IκB (inhibitor of NF-κB) kinase β IL interleukin UC ulcerative colitis medicine.anatomical_structure IL-34 Immunology LSD least significant difference Interleukin 34 Colitis Ulcerative Tumor necrosis factor alpha NF-κB nuclear factor κB medicine.symptom business |
| Zdroj: | Clinical Science (London, England : 1979) |
| ISSN: | 1470-8736 0143-5221 |
| DOI: | 10.1042/cs20150176 |
| Popis: | IBD (inflammatory bowel disease), where CD (Crohn's disease) and UC (ulcerative colitis) represent the two main forms, are chronic inflammatory conditions of the intestine. Macrophages play a central role in IBD pathogenesis and are regulated by major differentiation factors such as CSF-1 (colony-stimulating factor 1) in homoeostasis and inflammation. IL (interleukin)-34 has recently been discovered as a second ligand for CSF-1R (CSF-1 receptor). However, expression and involvement of IL-34 in IBD remain unknown. In the present paper, we investigated the expression of IL34, CSF1 and their shared receptor CSF1R in normal human ileum and colon, in inflamed and non-inflamed tissues of CD and UC patients, and in a mouse model of experimental colitis. We found distinct expression patterns of IL34 and CSF1 in ileum and colon, with higher IL34 in ileum and, in contrast, higher CSF1 in colon. Furthermore, IL34 and CSF1 expression was increased with inflammation in IBD patients and in experimental colitis. In humans, infiltrating cells of the lamina propria and intestinal epithelial cells expressed IL-34, and TNF-α (tumour necrosis factor α) regulated IL-34 expression in intestinal epithelial cells through the NF-κB (nuclear factor κB) pathway. These data demonstrate the expression pattern of IL-34 in ileum and colon and suggest IL-34 as a new modulator of inflammation in IBD. The macrophage differentiation factor interleukin-34, produced by intestinal epithelial cells, is up-regulated in patients with inflammatory bowel disease, and may be a novel modulator of intestinal inflammation. |
| Databáze: | OpenAIRE |
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