Porcine intact and wounded skin responses to atmospheric nonthermal plasma
| Autor: | R. Sensenig, Kristin Garcia, Suresh G. Joshi, Robert T. Ownbey, Ari D. Brooks, Essel Dulaimi, Danil Dobrynin, Krishna Priya Arjunan, Kenneth A. Barbee, Sameer Kalghatgi, Alexander Fridman, Erica R. Podolsky, Andrew Wu, Kimberly Wasko, Manjula Balasubramanian, Michelle Paff, Gary Friedman, Gregory Fridman, Ekaternia Cerchar |
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| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Pathology Time Factors Plasma Gases Swine Pilot Projects Wounds Penetrating Nonthermal plasma Histones Dermis medicine Animals Skin Wound Healing integumentary system Chemistry In vitro Acute toxicity Surgery Treatment Outcome medicine.anatomical_structure Coagulative necrosis Models Animal Toxicity Immunohistochemistry Female Laminin Wound healing |
| Zdroj: | Journal of Surgical Research. 179:e1-e12 |
| ISSN: | 0022-4804 |
| DOI: | 10.1016/j.jss.2012.02.039 |
| Popis: | Thermal plasma is a valued tool in surgery for its coagulative and ablative properties. We suggested through in vitro studies that nonthermal plasma can sterilize tissues, inactive pathogens, promote coagulation, and potentiate wound healing. The present research was undertaken to study acute toxicity in porcine skin tissues. We demonstrate that floating electrode-discharge barrier discharge (FE-DBD) nonthermal plasma is electrically safe to apply to living organisms for short periods. We investigated the effects of FE-DBD plasma on Yorkshire pigs on intact and wounded skin immediately after treatment or 24 h posttreatment. Macroscopic or microscopic histological changes were identified using histological and immunohistochemical techniques. The changes were classified into four groups for intact skin: normal features, minimal changes or congestive changes, epidermal layer damage, and full burn and into three groups for wounded skin: normal, clot or scab, and full burn-like features. Immunohistochemical staining for laminin layer integrity showed compromise over time. A marker for double-stranded DNA breaks, γ-H2AX, increased over plasma-exposure time. These findings identified a threshold for plasma exposure of up to 900 s at low power and |
| Databáze: | OpenAIRE |
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