A direct enzyme-linked immunosorbent assay (ELISA) for antibodies to enterobacterial Re core glycolipid and lipid A
| Autor: | François Damas, L. Joassin, Monique Nys, Pierre Damas, Jean Demonty |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Lipopolysaccharides
Male Microbiology (medical) medicine.medical_specialty Gram-negative bacteria Immunology Blood Donors Enzyme-Linked Immunosorbent Assay Biology Microbiology Lipid A Glycolipid Medical microbiology Salmonella Gram-Negative Bacteria medicine Animals Humans Immunology and Allergy In patient chemistry.chemical_classification Genetic Variation Bacterial Infections General Medicine biology.organism_classification Antibodies Bacterial Enzyme chemistry Antibody Formation biology.protein Female Rabbits Glycolipids Antibody Bacteria |
| Zdroj: | Medical Microbiology and Immunology. 176:257-271 |
| ISSN: | 1432-1831 0300-8584 |
| DOI: | 10.1007/bf00190532 |
| Popis: | We have developed an ELISA for IgM and IgG antibodies to the core glycolipid (CGL) of the Re mutant Salmonella minnesota R 595, and to lipid A. Anti-CGL antibodies have been detected in sera from 37% of healthy blood donors, whereas anti-lipid A activities were found in 13% of individuals only. The anti-CGL and anti-lipid A activities were examined in patients in a surgical intensive care unit, selected on the basis of a definite risk of infectious complications due to Gram-negative bacteria. Of the patients who developed such infections, the rate of favourable outcome was significantly higher in patients with either stable positive or increasing anti-CGL activities than in patients found to be negative. Our results provide clear evidence that anti-CGL antibodies contribute to host defence against various Gram-negative bacteria. |
| Databáze: | OpenAIRE |
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