Effect of Lippia alba and Cymbopogon citratus essential oils on biofilms of Streptococcus mutans and cytotoxicity in CHO cells
| Autor: | María C. Martínez-Pabón, Luciano Jose Merini, D. Galvis-Pareja, Mailen Ortega-Cuadros, H. Jiménez-Rios, Adriana Tofiño-Rivera |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
CIENCIAS MÉDICAS Y DE LA SALUD Acyclic Monoterpenes Cytotoxicity 030106 microbiology CHO Cells Microbial Sensitivity Tests Citral Dental plaque law.invention Microbiology Biotecnología de la Salud Cell Line Steam distillation Streptococcus mutans 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cricetulus Cymbopogon citratus law Drug Discovery medicine Oils Volatile Animals Plant Oils Cymbopogon Pharmacology biology Traditional medicine Terpenes ESENTIAL OILS Biofilm 030206 dentistry biology.organism_classification medicine.disease STREPTOCOCUS MUTANS chemistry Essential oils Biofilms Monoterpenes Dental caries BIOFILM Plant Preparations Lippia Lippia alba Geraniol Otras Biotecnologías de la Salud |
| Zdroj: | Repositorio Universidad de Santander Universidad de Santander instacron:Universidad de Santander |
| Popis: | 6 p. Background Caries is a public health problem, given that it prevails in 60 to 90% of the school-age global population. Multiple factors interact in its etiology, among them dental plaque is necessary to have lactic acid producing microorganisms like Streptococcus from he Mutans group. Existing prevention and treatment measures are not totally effective and generate adverse effects, which is why it is necessary to search for complementary strategies for their management. Aim The study sought to evaluate the eradication capacity of Streptococcus mutans biofilms and the toxicity on eukaryotic cells of Lippia alba and Cymbopogon citratus essential oils. Methodology Essential oils were extracted from plant material through steam distillation and then its chemical composition was determined. The MBEC-high-throughput (MBEC-HTP) (Innovotech, Edmonton, Alberta, Canada) assay used to determine the eradication concentration of S. mutans ATCC 35668 strain biofilms. Cytotoxicity was evaluated on CHO cells through the MTT cell proliferation assay. Results The major components in both oils were Geraniol and Citral; in L. alba 18.9% and 15.9%, respectively, and in C. citratus 31.3% and 26.7%. The L. alba essential oils presented eradication activity against S. mutans biofilms of 95.8% in 0.01 mg/dL concentration and C. citratus essential oils showed said eradication activity of 95.4% at 0.1, 0.01 mg/dL concentrations and of 93.1% in the 0.001 mg/dL concentration; none of the concentrations of both essential oils showed toxicity on CHO cells during 24 h. Conclusion The L. alba and C. citratus essential oils showed eradication activity against S. mutans biofilms and null cytotoxicity, evidencing the need to conduct further studies that can identify their active components and in order to guide a safe use in treating and preventing dental caries. Chemical compounds studied in this article List of up to 10 names of chemical compounds studied in the article. Geranial. Neral. Myrcene. Geraniol. (E)-Caryophyllene. trans-Verbenol. Geranyl acetate. cis-Verbenol. Germacrene D. 37-Dimethyl-26-octadiene-1-ol |
| Databáze: | OpenAIRE |
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