Effect of steviol, steviol glycosides and stevia extract on glucocorticoid receptor signaling in normal and cancer blood cells

Autor: Christina Panagiotou, Olga Katsarou, Paraskevi Moutsatsou, George Brauhli, Chrysovalantou Mihailidou
Rok vydání: 2017
Předmět:
0301 basic medicine
medicine.medical_specialty
Hydrocortisone
Cell Survival
Steviol
Biology
Response Elements
Biochemistry
Jurkat cells
Peripheral blood mononuclear cell
Dexamethasone
Tacrolimus Binding Proteins
03 medical and health sciences
chemistry.chemical_compound
Jurkat Cells
0302 clinical medicine
Endocrinology
Glucocorticoid receptor
Receptors
Glucocorticoid
Adrenocorticotropic Hormone
Glucosides
Internal medicine
Neoplasms
medicine
Humans
Stevia
Stevioside
RNA
Messenger
Luciferases
Molecular Biology
chemistry.chemical_classification
Cell Nucleus
Plant Extracts
Glycoside
medicine.disease
Subcellular localization
Leukemia
030104 developmental biology
chemistry
Gene Expression Regulation
030220 oncology & carcinogenesis
Leukocytes
Mononuclear
Diterpenes
Kaurane
Signal Transduction
Transcription Factors
Zdroj: Molecular and cellular endocrinology. 460
ISSN: 1872-8057
Popis: The use of steviol glycosides as non-caloric sweeteners has proven to be beneficial for patients with type 2 diabetes mellitus (T2D), obesity, and metabolic syndrome. However, recent data demonstrate that steviol and stevioside might act as glucocorticoid receptor (GR) agonists and thus correlate with adverse effects on metabolism. Herein, we evaluated the impact of steviol, steviol glycosides, and a Greek-derived stevia extract on a number of key steps of GR signaling cascade in peripheral blood mononuclear cells (PBMCs) and in Jurkat leukemia cells. Our results revealed that none of the tested compounds altered the expression of primary GR-target genes (GILZ, FKPB5), GR protein levels or GR subcellular localization in PBMCs; those compounds increased GILZ and FKPB5 mRNA levels as well as GRE-mediated luciferase activity, inducing in parallel GR nuclear translocation in Jurkat cells. The GR-modulatory activity demonstrated by stevia-compounds in Jurkat cells but not in PBMCs may be due to a cell-type specific effect.
Databáze: OpenAIRE
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