Important role of endocannabinoid signaling in the development of functional vision and locomotion in zebrafish

Autor: Rosa Maria Sepe, Paolo De Girolamo, Vincenzo Di Marzo, Oliana Carnevali, Andrea Martella, Giulia Fasano, Stephan C.F. Neuhauss, Cristoforo Silvestri, Paolo Sordino, Jingjing Zang
Přispěvatelé: Martella, Andrea, Sepe, Rosa M, Silvestri, Cristoforo, Zang, Jingjing, Fasano, Giulia, Carnevali, Oliana, DE GIROLAMO, Paolo, Neuhauss, Stephan C. F, Sordino, Paolo, Di Marzo, Vincenzo, University of Zurich
Rok vydání: 2016
Předmět:
0301 basic medicine
1303 Biochemistry
Cannabinoid receptor
2-Arachidonoylglycerol
Biochemistry
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
1311 Genetics
1312 Molecular Biology
Genetics
Animals
DAGL
Molecular Biology
Zebrafish
axonal pathfinding
Behavior
Animal
biology
Functional vision
musculoskeletal
neural
and ocular physiology
Axonal Pathfinding
Brain
Anatomy
biology.organism_classification
CB1
Endocannabinoid system
10124 Institute of Molecular Life Sciences
Axons
2-arachidonoylglycerol
Monoacylglycerol lipase
Lipoprotein Lipase
030104 developmental biology
nervous system
chemistry
1305 Biotechnology
MAGL
570 Life sciences
lipids (amino acids
peptides
and proteins)
Neuroscience
Locomotion
psychological phenomena and processes
030217 neurology & neurosurgery
Endocannabinoids
Signal Transduction
Biotechnology
Zdroj: The FASEB Journal. 30:4275-4288
ISSN: 1530-6860
0892-6638
Popis: The developmental role of the endocannabinoid system still remains to be fully understood. Here, we report the presence of a complete endocannabinoid system during zebrafish development and show that the genes that code for enzymes that catalyze the anabolism and catabolism (mgll and dagla) of the endocannabinoid, 2-AG (2-arachidonoylglycerol), as well as 2-AG main receptor in the brain, cannabinoid receptor type 1, are coexpressed in defined regions of axonal growth. By using morpholino-induced transient knockdown of the zebrafish Daglα homolog and its pharmacologic rescue, we suggest that synthesis of 2-AG is implicated in the control of axon formation in the midbrain-hindbrain region and that animals that lack Daglα display abnormal physiological behaviors in tests that measure stereotyped movement and motion perception. Our results suggest that the well-established role for 2-AG in axonal outgrowth has implications for the control of vision and movement in zebrafish and, thus, is likely common to all vertebrates.-Martella, A., Sepe, R. M., Silvestri, C., Zang, J., Fasano, G., Carnevali, O., De Girolamo, P., Neuhauss, S. C. F., Sordino, P., Di Marzo, V. Important role of endocannabinoid signaling in the development of functional vision and locomotion in zebrafish.
Databáze: OpenAIRE
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