Positron emission tomography study on pancreatic somatostatin receptors in normal and diabetic rats with 68Ga-DOTA-octreotide: A potential PET tracer for beta cell mass measurement
| Autor: | Yosky Kataoka, Yilong Cui, Yasuyoshi Watanabe, Yasuhiro Wada, Takeo Sako, Mie Nishimura, Koki Hasegawa, Michio Senda, Emi Hayashinaka, Yousuke Kanayama |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Biodistribution Biophysics Octreotide Cell Count Gallium Radioisotopes Biochemistry Rats Sprague-Dawley chemistry.chemical_compound Insulin-Secreting Cells Internal medicine Diabetes Mellitus Organometallic Compounds medicine Animals DOTA Receptors Somatostatin Radioactive Tracers Pancreas Molecular Biology Somatostatin receptor Cell Biology Streptozotocin Rats Endocrinology Somatostatin medicine.anatomical_structure chemistry Positron-Emission Tomography Beta cell medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 442:79-84 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2013.11.001 |
| Popis: | Diabetes mellitus (DM) is a metabolic disorder characterized by hyperglycemia, and the loss or dysfunction of pancreatic beta cells has been reported before the appearance of clinical symptoms and hyperglycemia. To evaluate beta cell mass (BCM) for improving the detection and treatment of DM at earlier stages, we focused on somatostatin receptors that are highly expressed in the pancreatic beta cells, and developed a positron emission tomography (PET) probe derived from octreotide, a metabolically stable somatostatin analog. Octreotide was conjugated with 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA), a chelating agent, and labeled with (68)Gallium ((68)Ga). After intravenous injection of (68)Ga-DOTA-octreotide, a 90-min emission scan of the abdomen was performed in normal and DM model rats. The PET studies showed that (68)Ga-DOTA-octreotide radioactivity was highly accumulated in the pancreas of normal rats and that the pancreatic accumulation was significantly reduced in the rats administered with an excess amount of unlabeled octreotide or after treatment with streptozotocin, which was used for the chemical induction of DM in rats. These results were in good agreement with the ex vivo biodistribution data. These results indicated that the pancreatic accumulation of (68)Ga-DOTA-octreotide represented specific binding to the somatostatin receptors and reflected BCM. Therefore, PET imaging with (68)Ga-DOTA-octreotide could be a potential tool for evaluating BCM. |
| Databáze: | OpenAIRE |
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