Secretion of cytokines by human macrophages upon infection by pathogenic and non-pathogenic mycobacteria
| Autor: | Eric Beltan, Nalin Rastogi, Lionel Horgen |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
musculoskeletal diseases
medicine.medical_treatment Enzyme-Linked Immunosorbent Assay In Vitro Techniques Microbiology Mycobacterium Heating Mycobacterium tuberculosis Immune system medicine Humans Macrophage Cells Cultured Mycobacterium Infections Virulence biology Interleukin-6 Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha Macrophages Granulocyte-Macrophage Colony-Stimulating Factor Interleukin biology.organism_classification Infectious Diseases Granulocyte macrophage colony-stimulating factor Cytokine Immunology Cytokines Tumor necrosis factor alpha Interleukin-1 medicine.drug |
| Zdroj: | Microbial Pathogenesis. 28:313-318 |
| ISSN: | 0882-4010 |
| Popis: | The interaction of various pathogenic (Mycobacterium tuberculosis, M. avium, M. kansasii, M. xenopi), and non-pathogenic mycobacteria (M. smegmatis, M. phlei) with human macrophages at the level of macrophage cytokine expression (TNFalpha, IL1, IL6 and GM-CSF) was investigated. Both for TNFalpha and GM-CSF, the lowest levels were obtained with pathogenic mycobacterial species, whereas about 2-8 times higher levels were observed for non-pathogenic species. Contrary to the above, the differences for IL6 and IL1 were not marked, although IL6 appeared to be more elevated for non-pathogenic species. Heat-killed bacteria induced a lower level of the cytokines for all the three cytokines assayed (TNFalpha, IL6 and IL1), except for M. tuberculosis for whom a significantly higher proportion of TNFalpha was induced by killed bacilli. The RT-PCR experiments performed on M. avium (as a low inducer of the cytokines) and M. smegmatis (as a high inducer of the cytokines) showed that the differences observed among pathogenic vs non-pathogenic strains were also reflected at the transcriptional level for TNFalpha and to a lesser extent for IL6, but not for IL1. This investigation underlined important differences existing between the pathogenic and non-pathogenic species, particularly as regards TNFalpha and GM-CSF. |
| Databáze: | OpenAIRE |
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