Skeletal myogenesis on highly orientated microfibrous polyesterurethane scaffolds
| Autor: | S.A. Riboldi, Peter Neuenschwander, L Pigini, P Mognol, Sara Mantero, Giulio Cossu, Maurilio Sampaolesi, M Marc Simonet, Nasser Sadr |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Scaffold
Materials science Polyesters Polyurethanes Biomedical Engineering Biocompatible Materials Muscle Development Cell Line Biomaterials Myoblasts MYOGENESIS Mice Tissue engineering Materials Testing medicine Cell Adhesion Myocyte Animals Cell adhesion Muscle Skeletal Tissue Engineering Myogenesis ELECTROSPINNING Metals and Alloys Skeletal muscle Adhesion Equipment Design TISSUE ENGINEERING Rats medicine.anatomical_structure FIBER ORIENTATION Ceramics and Composites SKELETAL MUSCLE C2C12 Biomedical engineering |
| Popis: | Skeletal myogenesis is a complex process, which is known to be intimately depending on an optimal outside-in substrate-cell signaling. Current attempts to reproduce skeletal muscle tissue in vitro using traditional scaffolds mainly suffer from poor directionality of the myofibers, resulting in an ineffective vectorial power generation. In this study, we aimed at investigating skeletal myogenesis on novel biodegradable microfibrous scaffolds made of DegraPol, a block polyesterurethane previously demonstrated to be suitable for this application. DegraPol was processed by electrospinning in the form of highly orientated ("O") and nonorientated ("N/O") microfibrous meshes and by solvent-casting in the form of nonporous films ("F"). The effect of the fiber orientation at the scaffold surface was evaluated by investigating C2C12 and L6 proliferation (via SEM analysis and alamarBlue test) and differentiation (via RT-PCR analysis and MHC immunostaining). We demonstrated that highly orientated elastomeric microfibrous DegraPol scaffolds enable skeletal myogenesis in vitro by aiding in (a) myoblast adhesion, (b) myotube alignment, and (c) noncoplanar arrangement of cells, by providing the necessary directional cues along with architectural and mechanical support. ispartof: Journal of Biomedical Materials Research A vol:84 issue:4 pages:1094-1101 ispartof: location:United States status: published |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|