Nitroxide-enhanced MRI of cardiovascular oxidative stress
| Autor: | Christopher D. Waters, Kenneth Walsh, Sophia X. Cui, Jonathan Leor, Heather Doviak, Ying Wang, Frederick H. Epstein, Brent A. French, Soham A Shah, Soichi Sano |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Tracer kinetic
Male medicine.medical_specialty Adenosine Pyrrolidines Saline infusion Myocardial Infarction medicine.disease_cause Diet High-Fat Cardiovascular System Article 030218 nuclear medicine & medical imaging Cyclic N-Oxides 03 medical and health sciences 0302 clinical medicine Dietary Sucrose Internal medicine medicine Animals Radiology Nuclear Medicine and imaging Myocardial infarction Obesity Spectroscopy business.industry Angiotensin II Reduction rate medicine.disease Magnetic Resonance Imaging Mice Inbred C57BL Perfusion Disease Models Animal Oxidative Stress Tissue sections Cardiology Molecular Medicine Nitrogen Oxides business Infarct zone 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | NMR Biomed |
| Popis: | BACKGROUND: In vivo imaging of oxidative stress can facilitate the understanding and treatment of cardiovascular diseases. We evaluated nitroxide-enhanced MRI with 3-carbamoyl-proxyl (3CP) for the detection of myocardial oxidative stress. METHODS: Three mouse models of cardiac oxidative stress were imaged, namely angiotensin II (Ang II) infusion, myocardial infarction (MI), and high-fat high-sucrose (HFHS) diet-induced obesity (DIO). For the Ang II model, mice underwent MRI at baseline and after 7 days of Ang II (n = 8) or saline infusion (n = 8). For the MI model, mice underwent MRI at baseline (n = 10) and at 1 (n = 8), 4 (n = 9), and 21 (n = 8) days after MI. For the HFHS-DIO model, mice underwent MRI at baseline (n = 20) and 18 weeks (n = 13) after diet initiation. The 3CP reduction rate, K(red), computed using a tracer kinetic model, was used as a metric of oxidative stress. Dihydroethidium (DHE) staining of tissue sections was performed on Day 1 after MI. RESULTS: For the Ang II model, K(red) was higher after 7 days of Ang II versus other groups (p < 0.05). For the MI model, K(red), in the infarct region was significantly elevated on Days 1 and 4 after MI (p < 0.05), whereas K(red) in the noninfarcted region did not change after MI. DHE confirmed elevated oxidative stress in the infarct zone on Day 1 after MI. After 18 weeks of HFHS diet, K(red) was higher in mice after diet versus baseline (p < 0.05). CONCLUSIONS: Nitroxide-enhanced MRI noninvasively quantifies tissue oxidative stress as one component of a multiparametric preclinical MRI examination. These methods may facilitate investigations of oxidative stress in cardiovascular disease and related therapies. |
| Databáze: | OpenAIRE |
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