Bioactive components and preventive effect of green tea (Camellia sinensis) extract on post-laparotomy intra-abdominal adhesion in rats
| Autor: | Mehrdad Karimi, Pouya Parsaei, Sayyed Yazdan Asadi, Mahmoud Rafieian-Kopaei |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Pathology medicine.medical_treatment Flavonoid Adhesion (medicine) Tissue Adhesions Abdominal cavity Green tea extract Gastroenterology Antioxidants Camellia sinensis Abdominal wall Random Allocation Peritoneum Phenols Internal medicine Laparotomy medicine Animals Rats Wistar chemistry.chemical_classification business.industry Plant Extracts Abdominal Cavity General Medicine Green tea medicine.disease Intra-abdominal adhesions Rats medicine.anatomical_structure chemistry Rat Surgery business |
| Zdroj: | International journal of surgery (London, England). 11(9) |
| ISSN: | 1743-9159 |
| Popis: | Background Adhesion formation is an important complication of abdomino-pelvic surgery. Green tea ( Camellia sinensis ) has anti-oxidant and anti-inflammatory effects which prevent production and accumulation of collagen and, thus, may reduce adhesion formation. The present study examined the effect of green tea alcoholic extract on intra-abdominal adhesion formation. Total phenolic, flavonoid and flavonol contents as well as anti-oxidant activity were also evaluated. Methods Thirty healthy male Wistar rats were randomly assigned to two equal groups of green tea (A) and distilled water (B). After anesthesia, the abdominal wall was opened and three shallow longitudinal and transverse incisions of 2 cm in length were made on the right side of the peritoneum by scalpel blade. A 2 × 2 cm square of the left abdominal wall peritoneum was removed by surgical scissors. Green tea extract or distilled water was introduced into the abdominal cavity of each rat. The rats were sacrificed two weeks post-laparotomy and adhesion bands were scored according to severity, extent and appearance. Fibrosis and inflammation were also scored via histopathological examination. Results There was a significant difference in mean adhesion scores between the green tea and distilled water groups (3.2 ± 3.503 and 7.33 ± 0.51, respectively) ( p = 0.001). In terms of fibrosis ( p = 0.002) and inflammation ( p = 0.003) a statistically significant difference was also seen between the two groups following histopathological examination. Conclusion Green tea extract reduces intra-peritoneal adhesions in an animal model. |
| Databáze: | OpenAIRE |
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