Molecular detection of SARS-COV-2 in exhaled breath at the point-of-need

Autor: Tim Stakenborg, Joren Raymenants, Ahmed Taher, Elisabeth Marchal, Bert Verbruggen, Sophie Roth, Ben Jones, Abdul Yurt, Wout Duthoo, Klaas Bombeke, Maarten Fauvart, Julien Verplanken, Rodrigo S. Wiederkehr, Aurelie Humbert, Chi Dang, Evi Vlassaks, Alejandra L. Jáuregui Uribe, Zhenxiang Luo, Chengxun Liu, Kirill Zinoviev, Riet Labie, Aduen Darriba Frederiks, Jelle Saldien, Kris Covens, Pieter Berden, Bert Schreurs, Joost Van Duppen, Rabea Hanifa, Megane Beuscart, Van Pham, Erik Emmen, Annelien Dewagtere, Ziduo Lin, Marco Peca, Youssef El Jerrari, Chinmay Nawghane, Chad Arnett, Andy Lambrechts, Paru Deshpande, Katrien Lagrou, Paul De Munter, Emmanuel André, Nik Van den Wijngaert, Peter Peumans
Přispěvatelé: Faculty of Engineering, Work and Organizational Psychology, Business, Psychology, Faculty of Sciences and Bioengineering Sciences, Faculty of Psychology and Educational Sciences
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: BIOSENSORS & BIOELECTRONICS
ISSN: 0956-5663
1873-4235
Popis: The SARS-CoV-2 pandemic has highlighted the need for improved technologies to help control the spread of contagious pathogens. While rapid point-of-need testing plays a key role in strategies to rapidly identify and isolate infectious patients, current test approaches have significant shortcomings related to assay limitations and sample type. Direct quantification of viral shedding in exhaled particles may offer a better rapid testing approach, since SARS-CoV-2 is believed to spread mainly by aerosols. It assesses contagiousness directly, the sample is easy and comfortable to obtain, sampling can be standardized, and the limited sample volume lends itself to a fast and sensitive analysis. In view of these benefits, we developed and tested an approach where exhaled particles are efficiently sampled using inertial impaction in a micromachined silicon chip, followed by an RT-qPCR molecular assay to detect SARS-CoV-2 shedding. Our portable, silicon impactor allowed for the efficient capture (>85%) of respiratory particles down to 300 nm without the need for additional equipment. We demonstrate using both conventional off-chip and in-situ PCR directly on the silicon chip that sampling subjects' breath in less than a minute yields sufficient viral RNA to detect infections as early as standard sampling methods. A longitudinal study revealed clear differences in the temporal dynamics of viral load for nasopharyngeal swab, saliva, breath, and antigen tests. Overall, after an infection, the breath-based test remains positive during the first week but is the first to consistently report a negative result, putatively signalling the end of contagiousness and further emphasizing the potential of this tool to help manage the spread of airborne respiratory infections. ispartof: BIOSENSORS & BIOELECTRONICS vol:217 ispartof: location:England status: published
Databáze: OpenAIRE
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